A new strategy to construct a FRET platform for ratiometric sensing of hydrogen sulfide

被引：111

作者：

He, Longwei ^{[1
]}

Lin, Weiying ^{[1
,2
]}

Xu, Qiuyan ^{[1
]}

Wei, Haipeng ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

[2] Univ Jinan, Sch Biol Sci & Technol, Sch Chem & Chem Engn, Inst Fluorescent Probes Biol Imaging, Jinan 250022, Shandong, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 08期

关键词：

2-PHOTON FLUORESCENT-PROBE; LIVING CELLS; BIOIMAGING APPLICATIONS; DESIGN; H2S; FLUOROPHORES;

D O I：

10.1039/c4cc08522a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We introduce a new FRET strategy to construct a ratiometric fluorescent H2S sensor. The ratio emission signal of the coumarin-naphthalimide dyad is modulated by the FRET process, which works in coordination with the ICT mechanism. The FRET process on/off is controlled through tuning the overlap level of the donor emission spectrum with the acceptor absorption via modulation of the acceptor fluorophore absorption wavelength. CN-N-3 was applied to visualize both the intracellular exogenous and endogenous H2S through blue and green emission channels.

引用

收藏

页码：1510 / 1513

页数：4

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