A DNA-EXTRACTION AND POLYMERASE-CHAIN-REACTION MICROCHIP USING MAGNETIC BEADS AND THERMO-PNEUMATIC VALVES

被引：5

作者：

Chia, Bonnie T. ^{[1
]}

Yang, Xing-Ying ^{[1
]}

Cheng, Ming-Yuan ^{[1
]}

Yang, Yao-Joe ^{[1
]}

机构：

[1] Natl Taiwan Univ, Taipei 10764, Taiwan

来源：

MEMS 2010: 23RD IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST | 2010年

关键词：

D O I：

10.1109/MEMSYS.2010.5442369

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

In this work, we develop a miniaturized system for DNA extraction and polymerase chain reaction (PCR). DNA is extracted from whole blood sample by using silica coated magnetic beads. Electromagnetic actuation is employed to manipulate the magnetic beads for transporting DNA molecules in fluidic channels. Thermo-pneumatic valves with low temperature elevation are integrated into the microfluidic chip for isolating/releasing fluidic samples and reagents. The size of the system is 72mmx54mmx11mm. The device fully operates with a 12V DC voltage and automatically performs DNA extraction and amplification processes after samples and reagents are loaded.

引用

页码：967 / 970

页数：4

共 6 条

[1] CHIA BT, 2009, TRANSD 09 C DENV COL, P2286
[2] A NOVEL MICROFLUIDIC PLATFORM FOR CONTINUOUS DNA EXTRACTION AND PURIFICATION USING LAMINAR FLOW MAGNETOPHORESIS
Karle, Marc
Miwa, Junichi
Roth, Guenter
Zengerle, Roland
von Stetten, Felix
[J]. IEEE 22ND INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2009), 2009, : 276 - 279
[3] Droplet-based DNA purification in a magnetic lab-on-a-chip
Lehmann, Ulrike
Vandevyver, Caroline
Parashar, Virendra K.
Gijs, Martin A. M.
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (19) : 3062 - 3067
[4] BEAD-BASED MICROFLUIDIC PLATFORM INTEGRATED WITH OPTICAL DETECTION DEVICES FOR RAPID DETECTION OF GENETIC DELETION FROM SALIVA
Lien, Kang-Yi
Liu, Chien-Ju
Kuo, Pao-Lin
Lee, Gwo-Bin
[J]. IEEE 22ND INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2009), 2009, : 340 - 343
[5] Clockwork PCR including sample preparation
Pipper, Juergen
Zhang, Yi
Neuzil, Pavel
Hsieh, Tseng-Ming
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (21) : 3900 - 3904
[6] Long-range transport of magnetic microbeads using simple planar coils placed in a uniform magnetostatic field
Rida, A
Fernandez, V
Gijs, MAM
[J]. APPLIED PHYSICS LETTERS, 2003, 83 (12) : 2396 - 2398

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