Tet-On induction with doxycycline after gene transfer in mice: Sweetening of drinking water is not a good idea

被引:15
作者
Hojman, Pernille [1 ]
Eriksen, Jens [1 ]
Gehl, Julie [1 ]
机构
[1] Univ Copenhagen, Herlev Hosp, Dept Oncol 5405, DK-2730 Herlev, Denmark
关键词
doxycycline; electroporation; gene expression; mice; sucrose; Tet-On system;
D O I
10.1080/10495390601105055
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Gene transfer to skeletal muscle leads to long-term, stable expression of transferred genes. An exiting development is the use of inducible expression systems. Using the inducible Tet-On system, it has been customary to administer doxycycline in drinking water with added sucrose to ameliorate the bitter taste. During a study aiming at regulating electro-transferred genes through the Tet-On system, we observed excessive drinking behavior among mice. Removal of sugar from the drinking water led to normal drinking behavior and most importantly did not affect the level of gene expression. Based on this study, the practice of adding sucrose to drinking water in doxycycline induction studies should be abandoned.
引用
收藏
页码:183 / 188
页数:6
相关论文
共 14 条
[1]   Use of the tetracycline system for inducible protein synthesis in the kidney [J].
Gallagher, AR ;
Schönig, K ;
Brown, N ;
Bujard, H ;
Witzgall, R .
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2003, 14 (08) :2042-2051
[2]   TRANSCRIPTIONAL ACTIVATION BY TETRACYCLINES IN MAMMALIAN-CELLS [J].
GOSSEN, M ;
FREUNDLIEB, S ;
BENDER, G ;
MULLER, G ;
HILLEN, W ;
BUJARD, H .
SCIENCE, 1995, 268 (5218) :1766-1769
[3]   Tetracycline-inducible system for regulation of skeletal muscle-specific gene expression in transgenic mice [J].
Grill, MA ;
Bales, MA ;
Fought, AN ;
Rosburg, KC ;
Munger, SJ ;
Antin, PB .
TRANSGENIC RESEARCH, 2003, 12 (01) :33-43
[4]   Early events of electroporation-mediated intramuscular DNA vaccination potentiate Thi-directed immune responses [J].
Gronevik, E ;
Mathiesen, I ;
Lomo, T .
JOURNAL OF GENE MEDICINE, 2005, 7 (09) :1246-1254
[5]   Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice [J].
Kistner, A ;
Gossen, M ;
Zimmermann, F ;
Jerecic, J ;
Ullmer, C ;
Lubbert, H ;
Bujard, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (20) :10933-10938
[6]   Construction of an rtTA2s-M2/tTSkid-based transcription regulatory switch that displays no basal activity, good inducibility, and high responsiveness to doxycycline in mice and non-human primates [J].
Lamartina, S ;
Silvi, L ;
Roscilli, G ;
Casimiro, D ;
Simon, AJ ;
Davies, ME ;
Shiver, JW ;
Rinaudo, CD ;
Zampaglione, I ;
Fattori, E ;
Colloca, S ;
Paz, OG ;
Laufer, R ;
Bujard, H ;
Cortese, R ;
Ciliberto, G ;
Toniatti, C .
MOLECULAR THERAPY, 2003, 7 (02) :271-280
[7]   Stringent control of gene expression in vivo by using novel doxycycline-dependent trans-activators [J].
Lamartina, S ;
Roscilli, G ;
Rinaudo, CD ;
Sporeno, E ;
Silvi, L ;
Hillen, W ;
Bujard, H ;
Cortese, R ;
Ciliberto, G ;
Toniatti, C .
HUMAN GENE THERAPY, 2002, 13 (02) :199-210
[8]   Coelectrotransfer to skeletal muscle of three plasmids coding for antiangiogenic factors and regulatory factors of the tetracycline-inducible system: Tightly regulated expression, inhibition of transplanted tumor growth, and antimetastatic effect [J].
Martel-Renoir, D ;
Trochon-Joseph, W ;
Galaup, A ;
Bouquet, C ;
Griscelli, F ;
Opolon, P ;
Opolon, D ;
Connault, E ;
Mir, L ;
Perricaudet, M .
MOLECULAR THERAPY, 2003, 8 (03) :425-433
[9]   Electric Pulse-Mediated Gene Delivery to Various Animal Tissues [J].
Mir, Lluis M. ;
Moller, Pernille H. ;
Andre, Franck ;
Gehl, Julie .
NON-VIRAL VECTORS FOR GENE THERAPY, SECOND EDITION: PART 2, 2005, 54 :83-114
[10]   Tetracycline transcriptional silencer tightly controls transgene expression after in vivo intramuscular electrotransfer:: Application to interleukin 10 therapy in experimental arthritis [J].
Perez, N ;
Plence, P ;
Millet, V ;
Greuet, D ;
Minot, C ;
Noel, D ;
Danos, O ;
Jorgensen, C ;
Apparailly, F .
HUMAN GENE THERAPY, 2002, 13 (18) :2161-2172