Metabolic transformations and characterisation of the sludge community in an enhanced biological phosphorus removal system

被引:53
作者
Christensson, M [1 ]
Blackall, LL
Welander, T
机构
[1] Univ Queensland, Dept Microbiol, Adv Wastewater Management Ctr, Brisbane, Qld 4072, Australia
[2] Univ Lund, Ctr Chem, Dept Biotechnol, S-22100 Lund, Sweden
关键词
D O I
10.1007/s002530051163
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Enhanced biological phosphorus removal was performed in a continuous laboratory-scale two-reactor system with sludge recirculation over a 75-day period. Influent wastewater was a synthetic medium based on acetate, and the sludge age was kept at 12 days. The adapted sludge stored poly-beta-hydroxyalkanoic acids (PHA) in the anaerobic reactor with a conversion ratio of 1.45 PHA/acetic acid (based on chemical O-2 demand: COD/COD) and gave ratio of a phosphate-P release to acetic acid uptake of 0.51 P/CH3COOH (w/w). Fractionation of anaerobic and aerobic sludges showed that the main part of phosphorus taken up, was eluted in the trichloroacetic acid fraction indicating that it was polyphosphate. A total of 60% of the phosphorus in the aerobic sludge was solubilized in the trichloroacetic acid fraction, whereas this fraction accounted for only 32% of the phosphorus in the anaerobic sludge. Only 4% of the total phosphorus in the aerobic sludge and 2% in the anaerobic sludge was found in the EDTA fraction, indicating low amounts of metal-bound phosphates. Isolation on acetate-based agar medium showed that Acinetobacter strains were present in the sludge. However, a more complete analysis of the bacterial community of the sludge was obtained by creating a clone library based on the 16S rRNA gene. A total of 51 partial clone sequences were phylogenetically evaluated. The predominating group was found in the high-(G + C) (mol%) gram-positive bacterial subphylum (31% of the sequenced clones), while the gamma proteobacteria only constituted 9.8% of the clones.
引用
收藏
页码:226 / 234
页数:9
相关论文
共 33 条
  • [1] [Anonymous], 1991, NUCL ACID TECHNIQUES
  • [2] APHA AWWA WEF, 1995, STAND METH EX WAT WA
  • [3] BAYLY RC, 1989, AWWA 13 FED CONV CAN, P162
  • [4] Blackall Linda L., 1994, Systematic and Applied Microbiology, V17, P513
  • [5] MOLECULAR-IDENTIFICATION OF ACTIVATED-SLUDGE FOAMING BACTERIA
    BLACKALL, LL
    [J]. WATER SCIENCE AND TECHNOLOGY, 1994, 29 (07) : 35 - 42
  • [6] The characterization and description of representatives of 'G' bacteria from activated sludge plants
    Blackall, LL
    Rossetti, S
    Christensson, C
    Cunningham, M
    Hartman, P
    Hugenholtz, P
    Tandoi, V
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 1997, 25 (01) : 63 - 69
  • [7] BLACKALL LL, 1987, THESIS U QUEENSLAND
  • [8] BACTERIAL COMMUNITY STRUCTURES OF PHOSPHATE-REMOVING AND NON-PHOSPHATE-REMOVING ACTIVATED SLUDGES FROM SEQUENCING BATCH REACTORS
    BOND, PL
    HUGENHOLTZ, P
    KELLER, J
    BLACKALL, LL
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (05) : 1910 - 1916
  • [9] Boumans P.W. J. M., 1987, INDUCTIVELY COUPLED
  • [10] BUCHAN L, 1981, WATER SA, V7, P1