A genome-wide resource for the analysis of protein localisation in Drosophila

被引：242

作者：

Sarov, Mihail ^{[1
]}

Barz, Christiane ^{[2
]}

Jambor, Helena ^{[1
]}

Hein, Marco Y. ^{[3
]}

Schmied, Christopher ^{[1
]}

Suchold, Dana ^{[1
]}

Stender, Bettina ^{[2
]}

Janosch, Stephan ^{[1
]}

Vikas, Vinay K. J. ^{[4
]}

Krisnan, R. T. ^{[4
]}

Krishnamoorthy, Aishwarya ^{[4
]}

Ferreira, Irene R. S. ^{[2
]}

Ejsmont, Radoslaw K. ^{[1
]}

Finkl, Katja ^{[2
]}

Hasse, Susanne ^{[1
]}

Kaempfer, Philipp ^{[5
]}

Plewka, Nicole ^{[2
]}

Vinis, Elisabeth ^{[1
]}

Schloissnig, Siegfried ^{[5
]}

Knust, Elisabeth ^{[1
]}

Hartenstein, Volker ^{[6
]}

Mann, Matthias ^{[3
]}

Ramaswami, Mani ^{[7
]}

VijayRaghavan, K. ^{[4
]}

Tomancak, Pavel ^{[1
]}

Schnorrer, Frank ^{[2
]}

机构：

[1] Max Planck Inst Cell Biol & Genet, Dresden, Germany

[2] Max Planck Inst Biochem, Muscle Dynam Grp, Klopferspitz 18A, D-82152 Martinsried, Germany

[3] Max Planck Inst Biochem, Dept Prot & Signal Transduct, Klopferspitz 18A, D-82152 Martinsried, Germany

[4] Tata Inst Fundamental Res, Ctr Cellular & Mol Platforms, Natl Ctr Biol Sci, Bangalore, Karnataka, India

[5] Heidelberg Inst Theoret Studies, Heidelberg, Germany

[6] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA USA

[7] Univ Dublin Trinity Coll, Inst Neurosci, Dublin 2, Ireland

来源：

ELIFE | 2016年 / 5卷

基金：

欧洲研究理事会;

关键词：

OSKAR MESSENGER-RNA; INDIRECT FLIGHT MUSCLES; FLUORESCENT-PROTEIN; TRANSGENIC RNAI; BINDING PROTEIN; EXPRESSION; VERSATILE; COMPLEX; REVEALS; IDENTIFICATION;

D O I：

10.7554/eLife.12068

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.

引用

页数：38

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