All-trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells

被引：25
作者：
Su, Zhong-yuan [1 ]
Li, Ying [2 ]
Zhao, Xiao-li [1 ]
Zhang, Ming [1 ]
机构：
[1] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Acad Med Sci, Hangzhou 310058, Zhejiang, Peoples R China
来源：
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B | 2010年 / 11卷 / 07期
关键词：
Bone marrow-derived mesenchymal stem cells; Smooth muscle cells; All-trans retinoic acid; Rabbit; IN-VITRO; PROLIFERATION;
D O I：
10.1631/jzus.B0900415
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bone marrow-derived mesenchymal stem cells are multipotent stem cells, an attractive resource for regenerative medicine. Accumulating evidence suggests that all-trans retinoic acid plays a key role in the development and differentiation of smooth muscle cells. In the present study, we demonstrate, for the first time, that rabbit bone marrow-derived mesenchymal stem cells differentiate into smooth muscle cells upon the treatment with all-trans retinoic acid. All-trans retinoic acid increased the expression of myocardin, caldesmon, 22-kDa smooth muscle cellspecific protein (SM22 alpha), and SM-myosin heavy chains in rabbit bone marrow-derived mesenchymal stem cells, as detected by reverse transcription polymerase chain reaction (PCR). Immunostaining of SM22 alpha and SM-myosin heavy chains using monoclonal antibodies also indicated smooth muscle cell differentiation of rabbit bone marrowderived mesenchymal stem cells following the treatment with all-trans retinoic acid. In addition, more than 47% of bone marrow-derived mesenchymal stem cells demonstrated the contractile phenotype of smooth muscle cells. Western blot results showed that SM-1 and SM-2 were highly expressed in the differentiated cells. These results suggest that all-trans retinoic acid may serve as a potent agent for functional smooth muscle cell differentiation in tissue engineering.
引用
收藏
页码：489 / 496
页数：8
相关论文
共 24 条
[1]   All-trans retinoic acid regulates proliferation, migration, differentiation, and extracellular matrix turnover of human arterial smooth muscle cells [J].
Axel, DI ;
Frigge, A ;
Dittmann, J ;
Runge, H ;
Spyridopoulos, I ;
Riessen, R ;
Viebahn, R ;
Karsch, KR .
CARDIOVASCULAR RESEARCH, 2001, 49 (04) :851-862
[2]   The tumorigenicity of human embryonic stem cells [J].
Blum, Barak ;
Benvenisty, Nissim .
ADVANCES IN CANCER RESEARCH, VOL 100, 2008, 100 :133-+
[3]   Mesenchymal progenitor cells differentiate into an endothelial phenotype, enhance vascular density, and improve heart function in a rat cellular cardiomyoplasty model [J].
Davani, S ;
Marandin, A ;
Mersin, N ;
Royer, B ;
Kantelip, B ;
Hervé, P ;
Etievent, JP ;
Kantelip, JP .
CIRCULATION, 2003, 108 (10) :253-258
[4]   Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli [J].
Di Nicola, M ;
Carlo-Stella, C ;
Magni, M ;
Milanesi, M ;
Longoni, PD ;
Matteucci, P ;
Grisanti, S ;
Gianni, AM .
BLOOD, 2002, 99 (10) :3838-3843
[5]   PHENOTYPIC CHANGES OF HUMAN SMOOTH-MUSCLE CELLS DURING DEVELOPMENT - LATE EXPRESSION OF HEAVY CALDESMON AND CALPONIN [J].
FRID, MG ;
SHEKHONIN, BV ;
KOTELIANSKY, VE ;
GLUKHOVA, MA .
DEVELOPMENTAL BIOLOGY, 1992, 153 (02) :185-193
[6]   Differentiation of human embryonic stem cells into smooth muscle cells in adherent monolayer culture [J].
Huang, Huarong ;
Zhao, Xiaoli ;
Chen, Liangbiao ;
Xu, Chao ;
Yao, Xing ;
Lu, Yongliang ;
Dai, Licheng ;
Zhang, Ming .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 351 (02) :321-327
[7]   Thromboxane A2 Induces Differentiation of Human Mesenchymal Stem Cells to Smooth Muscle-Like Cells [J].
Kim, Mi Ra ;
Jeon, Eun Su ;
Kim, Young Mi ;
Lee, Jung Sub ;
Kim, Jae Ho .
STEM CELLS, 2009, 27 (01) :191-199
[8]   All-trans retinoic acid inhibits vascular smooth muscle cell proliferation targeting multiple genes for cyclins and cyclin-dependent kinases. [J].
Kosaka, C ;
Sasaguri, T ;
Komiyama, Y ;
Takahashi, H .
HYPERTENSION RESEARCH, 2001, 24 (05) :579-588
[9]   Bone marrow mesenchymal stem cells inhibit the response of naive and memory antigen-specific T cells to their cognate peptide [J].
Krampera, M ;
Glennie, S ;
Dyson, J ;
Scott, D ;
Laylor, R ;
Simpson, E ;
Dazzi, F .
BLOOD, 2003, 101 (09) :3722-3729
[10]   HLA expression and immunologic properties of differentiated and undifferentiated mesenchymal stem cells [J].
Le Blanc, K ;
Tammik, C ;
Rosendahl, K ;
Zetterberg, E ;
Ringdén, O .
EXPERIMENTAL HEMATOLOGY, 2003, 31 (10) :890-896
123