Biocompatible electrochemiluminescent biosensor for choline based on enzyme/titanate nanotubes/chitosan composite modified electrode

被引：98

作者：

Dai, Hong ^{[1
,2
]}

Chi, Yuwu ^{[1
,2
]}

Wu, Xiaoping ^{[1
,2
]}

Wang, Youmei ^{[1
,2
]}

Wei, Mingdeng ^{[2
,3
]}

Chen, Guonan ^{[1
,2
]}

机构：

[1] Fuzhou Univ, Key Lab Anal & Detect Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350002, Fujian, Peoples R China

[2] Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China

[3] Fuzhou Univ, Inst Energy Technol & Nanomat, Fuzhou 350002, Fujian, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2010年 / 25卷 / 06期

关键词：

Electrochemiluminescence; Titanate nanotubes; Luminol; Choline oxidase; Choline; Biocompatibility; ELECTROGENERATED CHEMILUMINESCENT DETECTION; ALKANEBISPHOSPHONATE THIN-FILM; GLASS CARBON ELECTRODE; C-REACTIVE PROTEIN; TITANATE NANOTUBES; LUMINOL ELECTROCHEMILUMINESCENCE; HIGH AMPLIFICATION; INFANT FORMULAS; QUANTUM DOTS; NANOPARTICLES;

D O I：

10.1016/j.bios.2009.10.042

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A new biocompatible ECL biosensor based on enzyme/titanate nanotubes/chitosan composite film was developed for the determination of analytes in biological samples. In the fabrication of the new ECL biosensor, biocompatible titanate nanotubes (TNTs) and a model enzyme, i.e., choline oxidase (ChOX), were immobilized on a chitosan modified glassy carbon electrode (GCE) via electrostatic adsorption and covalent interaction, respectively. By this ECL biosensor, choline was enzymatically oxidized to hydrogen peroxide and detected by a sensitive luminol ECL system. The use of TNTs not only provided a biocompatible microenvironment for the immobilized enzyme, which resulted in an excellent stability and long lifetime of the ECL biosensor, but also exhibited great enhancement towards luminol ECL and thus led to a significant improvement in sensitivity of ECL biosensor. Satisfactory results were obtained when employing this biosensor in assaying the total choline in milk samples. The work would provide a common platform to develop various sensitive, selective and biocompatible ECL biosensors based on using enzyme/TNTs/CHIT composite films. (c) 2009 Elsevier B.V. All rights reserved.

引用

页码：1414 / 1419

页数：6

共 52 条

[1]

[Anonymous], J CATAL

[2]

[Anonymous], 2001, ELECTROCHEMICAL METH

[3] Nanostructured materials for electrochemiluminescence (ECL)-based detection methods: Recent advances and future perspectives [J].