Identification of cardiac glycosides in fractions from Periploca forrestii by high-performance liquid chromatography/diode-array detection/electrospray ionization multi-stage tandem mass spectrometry and liquid chromatography/nuclear magnetic resonance

被引:15
作者
Li, Yong
Wu, Xianfu
Li, Jianbei
Wang, Yinghong [1 ]
Yu, Shishan
Lv, Haining
Qu, Jing
Abliz, Zeper
Liu, Jing
Liu, Yuanyan
Du, Dan
机构
[1] Peking Union Med Coll, Inst Mat Med, Minist Educ, Key Lab Bioact Subst & Resources Utilizat Chinese, Beijing 100050, Peoples R China
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2010年 / 878卷 / 3-4期
关键词
Periploca forrestii; Cardiac glycosides; High-performance liquid chromatography/diode-array detection/electrospray ionization tandem mass spectrometry; Liquid chromatography/nuclear magnetic resonance; Structural characterization; RAPID STRUCTURAL DETERMINATION; CANCER; CARDENOLIDES; SAPONINS; OLEANDER; EXTRACT;
D O I
10.1016/j.jchromb.2009.12.008
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cardiac glycosides are a class of naturally Occurring Compounds that are characterized by some interesting biological activities and are widely distributed in the plant kingdom and can also be found in some animals. There is an interest in the chemical characterization of these molecules due to their toxicity and their use in medicines. In the Study reported here, a combination of electrospray ionization tandem mass spectrometry with high-performance liquid chromatography equipped with diode-array detector (HPLC-DAD/ESI-MSn), and hyphenation to both liquid chromatography and nuclear magnetic resonance spectroscopy (HPLC/NMR) were utilized for the on-line analyses of cardiac glycosides from Periploca forrestii, The fragmentation patterns and H-1 NMR spectra of nine isolated cardiac glycosides were investigated: their fragmentation rules and H-1 NMR spectral characteristics were summarized and applied to the structural identification of similar constituents in fractions from P. forrestii. As a result, a total of nine trace cardiac glycosides were tentatively determined by analyses of accurate molecular masses, representative fragment ions and characteristic H-1 NMR signals provided by HPLC/high-resolution mass spectrometry (HRMS), HPLC-DAD/ESI-MSn and HPLC/H-1 NMR experiments, respectively. Of these. eight (2-9) are new Compounds and one (1) is reported from P.forrestii for the first time. Results of the present study can benefit the rapid identification and targeted isolation of new cardiac glycosides from crude plant extracts. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:381 / 390
页数:10
相关论文
共 50 条
[41]   Characterization of polyphenol compounds from the roots and stems of Parthenocissus laetevirens by high-performance liquid chromatography/tandem mass spectrometry [J].
Chen, Juanjuan ;
He, Shan ;
Mao, Hui ;
Sun, Cuirong ;
Pan, Yuanjiang .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2009, 23 (06) :737-744
[42]   Evaluation of an extractionless high-performance liquid chromatography-tandem mass spectrometry method for detection and quantitation of rosiglitazone in canine plasma [J].
Frazier, Sara Allstadt ;
McKemie, Daniel S. ;
Guerrero, Teri A. ;
Skorupski, Katherine A. ;
Rodriguez, Carlos O., Jr. .
AMERICAN JOURNAL OF VETERINARY RESEARCH, 2011, 72 (02) :263-270
[43]   Comparison of gas chromatography-mass spectrometry and high-performance liquid chromatography with coulometric electrode array detection for determination of alkylresorcinol metabolites in human urine [J].
Marklund, Matti ;
Landberg, Rikard ;
Aman, Per ;
Kamal-Eldin, Afaf .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (9-10) :647-651
[44]   On line characterization of 58 phenolic compounds in Citrus fruit juices from Spanish cultivars by high-performance liquid chromatography with photodiode-array detection coupled to electrospray ionization triple quadrupole mass spectrometry [J].
Abad-Garcia, Beatriz ;
Garmon-Lobato, Sergio ;
Berrueta, Luis A. ;
Gallo, Blanca ;
Vicente, Francisca .
TALANTA, 2012, 99 :213-224
[45]   Identification and Quantification of Gingerols and Related Compounds in Ginger Dietary Supplements Using High-Performance Liquid Chromatography-Tandem Mass Spectrometry [J].
Tao, Yi ;
Li, Wenkui ;
Liang, Wenzhong ;
Van Breemen, Richard B. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (21) :10014-10021
[46]   Use of High-Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry for Structural Characterization of Bioactive Compounds in the Olive Root Bark and Wood of Chemlali Cultivar [J].
Ben Brahim, Samia ;
Priego-Capote, Feliciano ;
Bouaziz, Mohamed .
ACS OMEGA, 2022, :33873-33883
[47]   Identification of metabolites of geniposide in rat urine using ultra-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry [J].
Han, Han ;
Yang, Li ;
Xu, Ying ;
Ding, Yue ;
Bligh, S. W. Annie ;
Zhang, Tong ;
Wang, Zhengtao .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2011, 25 (21) :3339-3350
[48]   Diagnostic ion filtering strategy for chemical characterization of Guge Fengtong Tablet with high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry [J].
Zeng Su-Ling ;
Liu Xin-Guang ;
Lai Chang-Jiang-Sheng ;
Liu E-Hu ;
Li Ping .
CHINESE JOURNAL OF NATURAL MEDICINES, 2015, 13 (05) :390-400
[49]   A comprehensive strategy based on ultra-high performance liquid chromatography with diode array detector fingerprinting and multi-component ultra-performance liquid chromatography with tandem mass spectrometry technology for quality control of Jiawei Huoxiang Zhengqi Pill [J].
Lu, Yang ;
Lei, Ting ;
Chen, Xinyang ;
Ning, Na ;
Huang, Qiuling ;
Wu, Xu ;
Wang, Shengpeng ;
Li, Peng ;
Wan, Li ;
Cao, Jiliang .
JOURNAL OF SEPARATION SCIENCE, 2024, 47 (13)
[50]   Rapid method for simultaneous determination of flavonoid, saponins and polyacetylenes in Folium Ginseng and Radix Ginseng by pressurized liquid extraction and high-performance liquid chromatography coupled with diode array detection and mass spectrometry [J].
Qian, Z. M. ;
Lu, J. ;
Gao, Q. P. ;
Li, S. P. .
JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (18) :3825-3830