Specificity and Mechanism of Acinetobacter baumanii Nicotinamidase: Implications for Activation of the Front-Line Tuberculosis Drug Pyrazinamide

被引：44
作者：
Fyfe, Paul K. [1 ]
Rao, Vincenzo A. [1 ]
Zemla, Aleksandra [1 ]
Cameron, Scott [1 ]
Hunter, William N. [1 ]
机构：
[1] Univ Dundee, Coll Life Sci, Div Biol Chem & Drug Discovery, Dundee DD1 5EH, Scotland
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 48期
基金：
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词：
drug discovery; enzymes; mechanisms; tuberculosis; zinc; MYCOBACTERIUM-TUBERCULOSIS; CRYSTAL-STRUCTURE;
D O I：
10.1002/anie.200903407
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
TB or not TB active: The high-resolution crystal structures (see picture) of the zinccontaining metalloenzyme nicotinamidase in complex with nicotinic acid and pyrazinoic acid reveal new aspects of enzyme mechanism that help to explain the activation of a front-line anti-tuberculosis (TB) drug. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：9176 / 9179
页数：4
相关论文
共 13 条
  • [1] DeLano WL., 2002, PYMOL MOL GRAPHICS S
  • [2] Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii
    Du, XL
    Wang, WR
    Kim, R
    Yakota, H
    Nguyen, H
    Kim, SH
    [J]. BIOCHEMISTRY, 2001, 40 (47) : 14166 - 14172
  • [3] Leishmania trypanothione synthetase-amidase structure reveals a basis for regulation of conflicting synthetic and hydrolytic activities
    Fyfe, Paul K.
    Oza, Sandra L.
    Fairlamb, Alan H.
    Hunter, William N.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (25) : 17672 - 17680
  • [4] Geometry of metal-ligand interactions in proteins
    Harding, MM
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2001, 57 : 401 - 411
  • [5] Crystal structure of the yeast nicotinamidase Pnc1p
    Hu, Gang
    Taylor, Alexander B.
    McAlister-Henn, Lee
    Hart, P. John
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2007, 461 (01) : 66 - 75
  • [6] Characterization of new mutations in pyrazinamide-resistant strains of Mycobacterium tuberculosis and identification of conserved regions important for the catalytic activity of the pyrazinamidase PncA
    Lemaitre, N
    Sougakoff, W
    Truffot-Pernot, C
    Jarlier, V
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (07) : 1761 - 1763
  • [7] Magni G, 1999, ADV ENZYMOL RELAT AR, V73, P35
  • [8] Pace HC, 2001, GENOME BIOL, V2
  • [9] Overview of anti-tuberculosis (TB) drugs and their resistance mechanisms
    Shi, Ruiru
    Itagaki, Nobunori
    Sugawara, Isamu
    [J]. MINI-REVIEWS IN MEDICINAL CHEMISTRY, 2007, 7 (11) : 1177 - 1185
  • [10] Singh Pushpendra, 2006, J Commun Dis, V38, P288
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