Detection of parC mutations in Streptococcus pneumoniae by Real-time PCR and Taqman-MGB probes

被引：7
作者：
Alonso, Rodrigo
Mateo, Estibaliz
Cisterna, Ramon
机构：
[1] Univ Basque Country, Fac Farm, Dept Inmunol Microbiol & Parasitol, Vitoria 01006, Spain
[2] Hosp Basurto, Microbiol Serv, Bilbao 48013, Spain
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 69卷 / 01期
关键词：
fluoroquinolone; MGB probes; mutation real-time PCR; Streptococcus pneumoniae;
D O I：
10.1016/j.mimet.2006.12.016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A Real-time PCR assay was developed by using Taqman-MGB probes to screen mutations at codons Ser79 and Asp83 of Streptococcus pneuinoniae parC. One hundred and thirty levofloxacin-susceptible and forty-two levofloxacin-resistant clinical strains were assayed. Mutations at codon 79 were found among all the levofloxacin-resistant strains. Mutations at codon 79 or 83 were found in ten levofloxacin-susceptible strains. This procedure is a reliable method for a rapid detection of mutations in the QRDRs of parC gene of S. pneumoniae and could be carried out in a diagnostic laboratory for some high-risk patients or in epidemiological surveys. (c) 2007 Elsevier B.V. All rights reserved.
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收藏
页码：214 / 217
页数：4
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