Size exclusion chromatography - A blessing and a curse of science and technology of synthetic polymers

被引:128
作者
Berek, Dusan [1 ]
机构
[1] Slovak Acad Sci, Inst Polymer, Lab Liquid Chromatog, Bratislava 84236, Slovakia
关键词
Advantages; Benefits; Principles; Shortages and traps of size exclusion chromatography; Size exclusion chromatography; GEL-PERMEATION CHROMATOGRAPHY; OSMOTIC-PRESSURE CHROMATOGRAPHY; FIELD-FLOW FRACTIONATION; MOLECULAR-WEIGHT DISTRIBUTION; LIQUID-CHROMATOGRAPHY; HYDRODYNAMIC CHROMATOGRAPHY; ADSORPTION CHROMATOGRAPHY; CONCENTRATION-DEPENDENCE; CONFINED MACROMOLECULES; PREFERENTIAL SOLVATION;
D O I
10.1002/jssc.200900709
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Size exclusion chromatography, SEC is one of the most popular methods for the separation of different kinds of macromolecules. This critical review gives concise information about macromolecules and their behavior in solution, basic understanding about principles, instrumentation, and application possibilities of SEC, and more in detail discusses drawbacks and pitfalls of the method with the emphasis on synthetic polymers Selected practical advices are included to help enhance the quality of SEC results
引用
收藏
页码:315 / 335
页数:21
相关论文
共 50 条
[21]   Plate-model applied on concentration effects in size exclusion chromatography [J].
Netopilik, Milos .
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2020, 43 (15-16) :555-561
[22]   Size-Exclusion Chromatography: A Twenty-First Century Perspective [J].
Striegel, Andre M. .
CHROMATOGRAPHIA, 2022, 85 (04) :307-313
[23]   Accounting for shear scission in the size-exclusion chromatography of debranched starch [J].
Zhu, Jihui ;
Wang, Ziyi ;
Gilbert, Robert G. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2025, 417 (17) :3945-3957
[24]   Evaluation of multi-solvent size exclusion chromatography columns packed with sub-2 μm particles for the characterization of synthetic polymers [J].
Shen, Sensen ;
Bashir, Mubasher ;
Huysman, Steve ;
Xu, Xiuqing ;
Chang, Cuilan ;
Yang, Xiuhan ;
Pursch, Matthias ;
Chanaa, Sami ;
Byrd, Jade .
JOURNAL OF CHROMATOGRAPHY A, 2024, 1714
[25]   Quantitative analysis of star-branched polymers by multidetector size-exclusion chromatography [J].
Balke, ST ;
Mourey, TH ;
Robello, DR ;
Davis, TA ;
Kraus, A ;
Skonieczny, K .
JOURNAL OF APPLIED POLYMER SCIENCE, 2002, 85 (03) :552-570
[26]   Band broadening function in size exclusion chromatography of polymers:: Review of some recent developments [J].
Meira, Gregorio ;
Netopilik, Milog ;
Potschka, Martin ;
Schnoell-Bitai, Irene ;
Vega, Jorge .
MACROMOLECULAR SYMPOSIA, 2007, 258 :186-197
[27]   Considering the selectivity of pore size gradient size exclusion chromatography columns [J].
Fekete, Szabolcs ;
Lauber, Matthew ;
Xu, Mingcheng .
JOURNAL OF CHROMATOGRAPHY A, 2024, 1718
[28]   Size exclusion chromatography of aromatic compounds in high-boiling petroleum samples [J].
Panda, Saroj K. ;
Al-Zahrani, Abdullah A. ;
Kearney, Dominic J. ;
Altarooti, Enas S. ;
Al-Qunaysi, Thunayyan A. .
JOURNAL OF CHROMATOGRAPHY A, 2021, 1657
[29]   Size-exclusion chromatography with multi-angle static light scattering [J].
Striegel, Andre M. ;
Brewer, Amandaa K. ;
Zielke, Claudia .
NATURE REVIEWS METHODS PRIMERS, 2025, 5 (01)
[30]   The Critical Role of Mobile Phase Composition in Size Exclusion Chromatography of Protein Pharmaceuticals [J].
Arakawa, Tsutomu ;
Ejima, Daisuke ;
Li, Tiansheng ;
Phil, John S. .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2010, 99 (04) :1674-1692