Chimeric LNA/DNA probes as a detection system for real-time PCR

被引：31
作者：
Costa, JM
Ernault, P
Olivi, M
Gaillon, T
Arar, K
机构：
[1] Amer Hosp Paris, Ctr Diagnost Prenatal, M Dassault Mol Biol Lab, F-92200 Neuilly Sur Seine, France
[2] Proligo, F-75011 Paris, France
来源：
CLINICAL BIOCHEMISTRY | 2004年 / 37卷 / 10期
关键词：
real-time PCR; locked nucleic acid probe;
D O I：
10.1016/j.clinbiochem.2004.05.020
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objective: Evaluation of the use of short probes as a detection system in real-time PCR. Design and method: Comparison of results obtained with hybridization probes with and without locked nucleic acid (LNA) residues in the detection of fetal DNA in maternal serum. Results: The use of chimeric LNA/DNA probes led to a slight but significantly higher level of sensitivity as well as a higher level of fluorescence signal. Conclusion: Chimeric LNA/DNA probes offer an interesting alternative detection method in real-time PCR. (C) 2004 The Canadian Society of Clinical Chemists. All rights reserved.
引用
收藏
页码：930 / 932
页数：3
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