Indole acetonitrile-sensitivity of transgenic tobacco containing Arabidopsis thaliana nitrilase genes

被引：8

作者：

Dohmoto, M ^{[1
]}

Sano, J ^{[1
]}

Isaji, G ^{[1
]}

Yamaguchi, K ^{[1
]}

机构：

[1] Kanazawa Univ, Inst Gene Res, Kanazawa, Ishikawa 9200934, Japan

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 10期

关键词：

transgenic tobacco; Arabidopsis thaliana; nitrilase; NIT genes; indole-3-acetic acid;

D O I：

10.1007/s002990000218

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The Arabidopsis thaliana genome has four nitrilase (nitrile aminohydrolase, EC 3.5.5.1) genes (NIT1 to NIT4). These nitrilases catalyze hydrolysis of indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA). Growth of A. thaliana is inhibited by IAN probably due to hydrolysis of IAN to IAA, while the tobacco (Nicotiana tabacum) genome has only NIT4 homologs and is resistant-to IAN. In this study, we introduced A. thaliana NIT1 to NIT4 into tobacco. Introduction of NIT1, NIT2 or NIT3 into tobacco conferred growth inhibition by IAN. NIT2 transgenic plants were highly sensitive to IAN, and NIT1 and NIT3 transgenic plants were moderately sensitive. On the other hand, NIT4 transgenic plants were less sensitive to IAN, although some morphological changes in the roots were observed as the wild-type tobacco. These findings suggest that the ability of transgenic tobacco to convert IAN to IAA in vivo is markedly different among transgenes of NIT1 to NIT4.

引用

页码：1027 / 1032

页数：6

共 16 条

[1] DIFFERENTIAL REGULATION OF AN AUXIN-PRODUCING NITRILASE GENE FAMILY IN ARABIDOPSIS-THALIANA [J].

BARTEL, B ;

FINK, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (14) :6649-6653

[2] CLONING AND EXPRESSION OF AN ARABIDOPSIS NITRILASE WHICH CAN CONVERT INDOLE-3-ACETONITRILE TO THE PLANT HORMONE, INDOLE-3-ACETIC-ACID [J].

BARTLING, D ;

SEEDORF, M ;

MITHOFER, A ;

WEILER, EW .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 205 (01) :417-424

[3] MOLECULAR CHARACTERIZATION OF 2 CLONED NITRILASES FROM ARABIDOPSIS-THALIANA - KEY ENZYMES IN BIOSYNTHESIS OF THE PLANT HORMONE INDOLE-3-ACETIC-ACID [J].

BARTLING, D ;

SEEDORF, M ;

SCHMIDT, RC ;

WEILER, EW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) :6021-6025

[4] BINARY AGROBACTERIUM VECTORS FOR PLANT TRANSFORMATION [J].

BEVAN, M .

NUCLEIC ACIDS RESEARCH, 1984, 12 (22) :8711-8721

[5] BIOSYNTHESIS AND METABOLISM OF INDOL-3YL-ACETIC ACID .2. IN-VIVO EXPERIMENTS WITH C-14-LABELED PRECURSORS OF IAA IN TOMATO AND BARLEY SHOOTS [J].

GIBSON, RA ;

WIGHTMAN, F ;

SCHNEIDER, EA .

JOURNAL OF EXPERIMENTAL BOTANY, 1972, 23 (75) :381-+

[6] A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS [J].

HORSCH, RB ;

FRY, JE ;

HOFFMANN, NL ;

EICHHOLTZ, D ;

ROGERS, SG ;

FRALEY, RT .

SCIENCE, 1985, 227 (4691) :1229-1231

[7]

JEFFERSON RA, 1987, EMBO J, V6, P3901

[8] A PLASMA MEMBRANE-BOUND ENZYME OXIDIZES L-TRYPTOPHAN TO INDOLE-3-ACETALDOXIME [J].

LUDWIGMULLER, J ;

HILGENBERG, W .

PHYSIOLOGIA PLANTARUM, 1988, 74 (02) :240-250

[9] Homology-dependent gene silencing in plants [J].

Meyer, P ;

Saedler, H .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1996, 47 :23-48

[10] Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants [J].

Mitsuhara, I ;

Ugaki, M ;

Hirochika, H ;

Ohshima, M ;

Murakami, T ;

Gotoh, Y ;

Katayose, Y ;

Nakamura, S ;

Honkura, R ;

Nishimiya, S ;

Ueno, K ;

Mochizuki, A ;

Tanimoto, H ;

Tsugawa, H ;

Otsuki, Y ;

Ohashi, Y .

PLANT AND CELL PHYSIOLOGY, 1996, 37 (01) :49-59

← 1 2 →