Identification of Pathogenic Rare Yeast Species in Clinical Samples: Comparison between Phenotypical and Molecular Methods

被引:52
作者
Cendejas-Bueno, Emilio [1 ]
Gomez-Lopez, Alicia [1 ]
Mellado, Emilia [1 ]
Rodriguez-Tudela, Juan L. [1 ]
Cuenca-Estrella, Manuel [1 ]
机构
[1] Inst Salud Carlos III, Ctr Nacl Microbiol, Serv Micol, Madrid 28220, Spain
关键词
AMPHOTERICIN-B; CANDIDA-ORTHOPSILOSIS; ANTIFUNGAL AGENTS; SP NOV; RESISTANT; OUTBREAK; SURVEILLANCE; METAPSILOSIS; FLUCONAZOLE; FUNGEMIA;
D O I
10.1128/JCM.00336-10
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Species identification using both phenotypic and molecular methods and antifungal susceptibility tests was carried out with 60 uncommon clinical yeasts. Our data show that phenotypic methods were insufficient for correct identification (only 25%) and that most of the wrongly identified strains showed a resistant antifungal profile.
引用
收藏
页码:1895 / 1899
页数:5
相关论文
共 26 条
[1]   Phenotypic and molecular characterization of Candida nivarzensis sp nov., a possible new opportunistic fungus [J].
Alcoba-Flórez, J ;
Méndez-Alvarez, S ;
Cano, J ;
Guarro, J ;
Pérez-Roth, E ;
Arévalo, MD .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (08) :4107-4111
[2]   Sequence-Based Identification of Aspergillus, Fusarium, and Mucorales Species in the Clinical Mycology Laboratory: Where Are We and Where Should We Go from Here? [J].
Balajee, S. A. ;
Borman, A. M. ;
Brandt, M. E. ;
Cano, J. ;
Cuenca-Estrella, M. ;
Dannaoui, E. ;
Guarro, J. ;
Haase, G. ;
Kibbler, C. C. ;
Meyer, W. ;
O'Donnell, K. ;
Petti, C. A. ;
Rodriguez-Tudela, J. L. ;
Sutton, D. ;
Velegraki, A. ;
Wickes, B. L. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (04) :877-884
[3]   Outbreak of Pichia anomala infection in the pediatric service of a tertiary-care center in Northern India [J].
Chakrabarti, A ;
Singh, K ;
Narang, A ;
Singhi, S ;
Batra, R ;
Rao, KLN ;
Ray, P ;
Gopalan, S ;
Das, S ;
Gupta, V ;
Gupta, AK ;
Bose, SM ;
McNeil, MM .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (05) :1702-1706
[4]   Outbreak of Candida rugosa candidemia:: an emerging pathogen that may be refractory to amphotericin B therapy [J].
Colombo, AL ;
Melo, ASA ;
Rosas, RFC ;
Salomao, R ;
Briones, MRS ;
Hollis, RJ ;
Messer, SA ;
Pfaller, MA .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2003, 46 (04) :253-257
[5]   Candida bracarensis sp nov., a novel anamorphic yeast species phenotypically similar to Candida glabrata [J].
Correia, A ;
Sampaio, P ;
James, S ;
Pais, C .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006, 56 :313-317
[6]   Head-to-head comparison of the activities of currently available antifungal agents against 3,378 Spanish clinical isolates of yeasts and filamentous fungi [J].
Cuenca-Estrella, M ;
Gomez-Lopez, A ;
Mellado, E ;
Buitrago, MJ ;
Monzon, A ;
Rodriguez-Tudela, JL .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2006, 50 (03) :917-921
[7]   In vitro susceptibilities of bloodstream isolates of Candida species to six antifungal agents:: results from a population-based active surveillance programme, Barcelona, Spain, 2002-2003 [J].
Cuenca-Estrella, M ;
Rodriguez, D ;
Almirante, B ;
Morgan, J ;
Planes, AM ;
Almela, M ;
Mensa, J ;
Sanchez, F ;
Ayats, J ;
Gimenez, M ;
Salvado, M ;
Warnock, DW ;
Pahissa, A ;
Rodriguez-Tudela, JL .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 55 (02) :194-199
[8]   Kodamaea (Pichia) ohmeri fungemia in a pediatric patient admitted in a public hospital [J].
De Barros, Jadson Duque ;
Nogueira Do Nascimento, Suerda Maria ;
Silva De Araujo, Fernanda Janaina ;
Dos Santos Braz, Regina De Fatima ;
Andrade, Vania Sousa ;
Theelen, Bart ;
Boekhout, Teun ;
Illnait-Zaragozi, Maria Teresa ;
Guimaraes Gouveia, Maria Narriman ;
Fernandes, Maria Conceicao ;
Lins Monteiro, Maria Goretti ;
Barreto De Oliveira, Maria Tereza .
MEDICAL MYCOLOGY, 2009, 47 (07) :775-779
[9]   Prevalence and susceptibility profile of Candida metapsilosis and Candida orthopsilosis:: Results from population-based surveillance of candidemia in Spain [J].
Gomez-Lopez, A. ;
Alastruey-Izquierdo, A. ;
Rodriguez, D. ;
Almirante, B. ;
Pahissa, A. ;
Rodriguez-Tudela, J. L. ;
Cuenca-Estrella, A. .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (04) :1506-1509
[10]   Candida ciferrii, a new fluconazole-resistant yeast causing systemic mycosis in immunocompromised patients [J].
Gunsilius, E ;
Lass-Flörl, C ;
Kähler, CM ;
Gastl, G ;
Petzer, AL .
ANNALS OF HEMATOLOGY, 2001, 80 (03) :178-179