A novel 8.7 kDa protease inhibitor from chan seeds (Hyptis suaveolens L.) inhibits proteases from the larger grain borer Prostephanus truncatus (Coleoptera: Bostrichidae)

被引：23

作者：

Aguirre, C

Valdés-Rodríguez, S

Mendoza-Hernández, G

Rojo-Domínguez, A

Blanco-Labra, A

机构：

[1] CINVESTAV, Unidad Irapuato, Dept Biotecnol & Bioquim, Irapuato 36500, Gto, Mexico

[2] Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City 04510, DF, Mexico

[3] Univ Autonoma Metropolitana Iztapalapa, Dept Quim, Iztapalapa 09340, Mexico

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2004年 / 138卷 / 01期

关键词：

chan; Hyptis suaveolens; larger grain borer; maize; Manduca sexta; plant defense mechanisms; Prostephanus truncatus; proteases; protease inhibitor;

D O I：

10.1016/j.cbpc.2004.02.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel trypsin inhibitor purified from chan seeds (Hyptis suaveolens, Lamiaceae) was purified and characterized. Its apparent molecular mass was 8700 Da with an isoelectric point of 3.4. Its N-terminal sequence showed a high content of acidic amino acids (seven out of 18 residues). Its inhibitory activity was potent toward all trypsin-like proteases extracted from the gut of the insect Prostephanus truncatus (Coleoptera: Bostrichidae), a very important pest of maize. This activity was highly specific, because among proteases from seven different insects, only those from P. truncatus and Manduca sexta (Lepidoptera: Sphingidae) were inhibited. This inhibitor has potential to enhance the defense mechanism of maize against the attack of P. truncatus. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：81 / 89

页数：9

共 37 条

[1] Transgenic expression of trypsin inhibitor CMe from barley in indica and japonica rice, confers resistance to the rice weevil Sitophilas oryzae [J].