Saccharopolyspora halotolerans sp nov., a halophilic actinomycete isolated from a hypersaline lake

A novel actinomycete strain, designated TRM 45123(T), was isolated from a hypersaline habitat in Xinjiang Province (40 degrees 20' N 90 degrees 49' E), north-west China. The isolate was characterized using a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain TRM 45123(T) belonged to the genus Saccharopolyspora and was closely related to Saccharopolyspora gloriosae (96.7 % similarity). The G+C content of the DNA was 69.07 mol%. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid, and arabinose and ribose as the major whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine and phosphatidylglycerol. The predominant menaquinone was MK-9(H-4). The major fatty acids were iso-C-16:0, anteiso-C-17:0, iso-C-15:0 and anteiso-C-15:0. On the basis of the evidence from this polyphasic study, a novel species, Saccharopolyspora halotolerans sp. nov., is proposed. The type strain of Saccharopolyspora halotolerans is TRM 45123(T) (=CCTCC AA 2013006(T)= DSM 45990(T)).