Pseudomonas bijieensis sp. nov., isolated from cornfield soil

Strain L22-9(T), a Gram-stain--negative and rod-shaped bacterium, motile by one polar flagellum, was isolated from cornfield soil in Bijie, Guizhou Province, PR China. Based on 16S rRNA gene sequences, it was identified as a Pseudomonas species. Multilocus sequence analysis of concatenated 16S rRNA, gyrB, rpoB and rpoD gene sequences showed that strain L22-9(T) formed a clearly separated branch, located in a cluster together with Pseudomonas brassicacearum LMG 21623(T), Pseudomonas kilonensis DSM 13647(T) and Pseudomonas thivervalensis DSM 13194(T). Whole-genome comparisons based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) confirmed that strain L22-9(T) should be classified as a novel species. It was most closely related to P. kilonensis DSM 13647(T) with ANI and dDDH values of 91.87 and 46.3%, respectively. Phenotypic features that can distinguish strain L22-9(T) from P. kilonensis DSM 13647(T) are the assimilation ability of N-acetyl-d-glucosamine, poor activity of arginine dihydrolase and failure to ferment ribose and d-fucose. The predominant cellular fatty acids of strain L22-9(T) are C-16:0, summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c) and summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c). The respiratory quinones consist of Q-9 and Q-8. The polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, two unidentified phosphoglycolipids, two unidentified aminophospholipids and an unidentified glycolipid. Based on the evidence, we conclude that strain L22-9(T) represents a novel species, for which the name Pseudomonas bijieensis sp. nov. is proposed. The type strain is L22-9(T) (=CGMCC 1.18528(T)=LMG 31948(T)), with a DNA G+C content of 60.85 mol%.