Dynamic control of plant water use using designed ABA receptor agonists

被引：129

作者：

Vaidya, Aditya S. ^{[1
,2
]}

Helander, Jonathan D. M. ^{[1
,2
]}

Peterson, Francis C. ^{[3
]}

Elzinga, Dezi ^{[1
,2
]}

Dejonghe, Wim ^{[1
,2
]}

Kaundal, Amita ^{[1
,4
]}

Park, Sang-Youl ^{[1
,2
]}

Xing, Zenan ^{[1
,2
]}

Mega, Ryousuke ^{[5
]}

Takeuchi, Jun ^{[6
,7
]}

Khanderahoo, Bardia ^{[1
,2
]}

Bishay, Steven ^{[1
,2
]}

Volkman, Brian F. ^{[3
]}

Todoroki, Yasushi ^{[6
,7
]}

Okamoto, Masanori ^{[8
,9
]}

Cutler, Sean R. ^{[1
,2
]}

机构：

[1] Univ Calif Riverside, Inst Integrat Genome Biol, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA

[3] Med Coll Wisconsin, Dept Biochem, Milwaukee, WI 53226 USA

[4] Utah State Univ, Dept Plants Soils & Climate, Logan, UT 84322 USA

[5] Tottori Univ, Arid Land Res Ctr, 1390 Hamasaka, Tottori 6800001, Japan

[6] Shizuoka Univ, Fac Agr, Shizuoka 4228529, Japan

[7] Shizuoka Univ, Res Inst Green Sci & Technol, Shizuoka 4228529, Japan

[8] Utsunomiya Univ, Ctr Biosci Res & Educ, 350 Mine, Utsunomiya, Tochigi 3218505, Japan

[9] Japan Sci & Technol Agcy, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan

来源：

SCIENCE | 2019年 / 366卷 / 6464期

基金：

美国国家科学基金会;

关键词：

ABSCISIC-ACID RECEPTORS; SELECTIVE ACTIVATION; STRUCTURE VALIDATION; DROUGHT TOLERANCE; MECHANISM; BINDING; LIGAND; ASSOCIATION; MOLPROBITY; INSIGHTS;

D O I：

10.1126/science.aaw8848

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Drought causes crop losses worldwide, and its impact is expected to increase as the world warms. This has motivated the development of small-molecule tools for mitigating the effects of drought on agriculture. We show here that current leads are limited by poor bioactivity in wheat, a widely grown staple crop, and in tomato. To address this limitation, we combined virtual screening, x-ray crystallography, and structure-guided design to develop opabactin (OP), an abscisic acid (ABA) mimic with up to an approximately sevenfold increase in receptor affinity relative to ABA and up to 10-fold greater activity in vivo. Studies in Arabidopsis thaliana reveal a role of the type III receptor PYRABACTIN RESISTANCE-LIKE 2 for the antitranspirant efficacy of OP. Thus, virtual screening and structure-guided optimization yielded newly discovered agonists for manipulating crop abiotic stress tolerance and water use.

引用

页码：446 / +

页数：56

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