Label-free evanescent microscopy for membrane nano-tomography in living cells

被引：5

作者：

Bon, Pierre ^{[1
,2
]}

Barroca, Thomas ^{[1
]}

Leveque-Fort, Sandrine ^{[2
]}

Fort, Emmanuel ^{[1
]}

机构：

[1] ESPCI ParisTech, CNRS, Inst Langevin, UMR 7587, F-75231 Paris 05, France

[2] Univ Paris 11, CNRS, ISMO, UMR 8214, F-91405 Orsay, France

来源：

JOURNAL OF BIOPHOTONICS | 2014年 / 7卷 / 11-12期

关键词：

evanescent field; membrane imaging; super-resolution; label-free samples; multilayer Fresnel model; Total Internal Reflection microscopy;

D O I：

10.1002/jbio.201300095

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that through-the-objective evanescent microscopy (epi-EM) is a powerful technique to image membranes in living cells. Readily implementable on a standard inverted microscope, this technique enables full-field and real-time tracking of membrane processes without labeling and thus signal fading. In addition, we demonstrate that the membrane/interface distance can be retrieved with 10 nm precision using a multilayer Fresnel model. We apply this nano-axial tomography of living cell membranes to retrieve quantitative information on membrane invagination dynamics. ((c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

引用

页码：857 / 862

页数：6

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