Real-time PCR for differential determination of the tomato wilt fungus, Fusarium oxysporum f. sp lycopersici, and its races

被引：33

作者：

Inami, Keigo ^{[1
]}

Yoshioka, Chizu ^{[1
]}

Hirano, Yasushi ^{[2
]}

Kawabe, Masato ^{[3
]}

Tsushima, Seiya ^{[4
]}

Teraoka, Tohru ^{[1
]}

Arie, Tsutomu ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Plant Pathol Lab, Fuchu, Tokyo 1838509, Japan

[2] Saitama Prefectural Agr & Forestry Res Ctr, Kuki, Saitama 3460037, Japan

[3] Natl Agr Res Ctr, Tsukuba, Ibaraki 305, Japan

[4] Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 305, Japan

来源：

JOURNAL OF GENERAL PLANT PATHOLOGY | 2010年 / 76卷 / 02期

关键词：

Avirulence gene; Detection; rDNA-intergenic spacer (IGS) region; Soil DNA; POLYMERASE-CHAIN-REACTION; SOIL; QUANTIFICATION; PATHOGENS; PLANT;

D O I：

10.1007/s10327-010-0224-7

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Five primer/probe sets to identify the tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici (FOL), and its three races selectively were designed based on the rDNA-intergenic spacer and avirulence genes. Real-time PCR using genomic DNA from mycelia and soil DNA with the primer/probe sets allowed the successful identification of FOL and its races.

引用

页码：116 / 121

页数：6

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