Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes

被引：32
作者：
Barraza-Garza, Guillermo [1 ]
Castillo-Michel, Hiram [2 ]
de la Rosa, Laura A. [1 ]
Martinez-Martinez, Alejandro [1 ]
Perez-Leon, Jorge A. [1 ]
Cotte, Marine [2 ]
Alvarez-Parrilla, Emilio [1 ]
机构：
[1] Univ Autonoma Ciudad Juarez, Inst Ciencias Biomed, Dept Ciencias Quim Biol, Ciudad Juarez 32310, CHIH, Mexico
[2] European Synchrotron Radiat Facil, Xray & Infrared Microspectroscopy Beamline ID21, BP 220, F-38043 Grenoble, France
来源：
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | 2016年 / 2016卷
关键词：
MOLECULAR-MECHANISMS; LIPID-PEROXIDATION; OXIDATIVE STRESS; SMALL-INTESTINE; CELLS; MICROSPECTROSCOPY; DISEASE; FLAVONOIDS; MICROSCOPY; ACID;
D O I：
10.1155/2016/9245150
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The protective effect of different polyphenols, catechin (Cat), quercetin (Qc) (flavonoids), gallic acid (GA), caffeic acid (CfA), chlorogenic acid (ChA) (phenolic acids), and capsaicin (Cap), against H2O2-induced oxidative stress was evaluated in rat enterocytes using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) Spectroscopy and Fourier Transform Infrared Microspectroscopy (FTIRM), and results were compared to standard lipid peroxidation techniques: conjugated dienes (CD) and Thiobarbituric Acid Reactive Substances (TBARS). Analysis of ATR-FTIR and FTIRM spectral data allowed the simultaneous evaluation of the effects of H2O2 and polyphenols on lipid and protein oxidation. All polyphenols showed a protective effect against H2O2-induced oxidative stress in enterocytes, when administered before or after H2O2. Cat and capsaicin showed the highest protective effect, while phenolic acids had weaker effects and Qc presented a mild prooxidative effect (IR spectral profile of biomolecules between control and H2O2-treated cells) according to FTIR analyses. These results demonstrated the viability to use infrared spectroscopy to evaluate the oxidant and antioxidant effect of molecules in cell systems assays.
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页数：10
相关论文
共 36 条
[1]   Protective effect of fresh and processed Jalapeno and Serrano peppers against food lipid and human LDL cholesterol oxidation [J].
Alvarez-Parrilla, Emilio ;
de la Rosa, Laura A. ;
Amarowicz, Ryszard ;
Shahidi, Fereidoon .
FOOD CHEMISTRY, 2012, 133 (03) :827-834
[2]   Structure of Inclusions of Huntington's Disease Brain Revealed by Synchrotron Infrared Microspectroscopy: Polymorphism and Relevance to Cytotoxicity [J].
Andre, William ;
Sandt, Christophe ;
Dumas, Paul ;
Djian, Philippe ;
Hoffner, Guylaine .
ANALYTICAL CHEMISTRY, 2013, 85 (07) :3765-3773
[3]   Molecular mechanisms of the cardiovascular protective effects of polyphenols [J].
Andriantsitohaina, Ramaroson ;
Auger, Cyril ;
Chataigneau, Thierry ;
Etienne-Selloum, Nelly ;
Li, Huige ;
Martinez, M. Carmen ;
Schini-Kerth, Valerie B. ;
Laher, Ismail .
BRITISH JOURNAL OF NUTRITION, 2012, 108 (09) :1532-1549
[4]  
[Anonymous], POLYPHENOLS HUMAN HL
[5]  
Bellisola G, 2012, AM J CANCER RES, V2, P1
[6]   Microspectroscopy (μFTIR) Reveals Co-localization of Lipid Oxidation and Amyloid Plaques in Human Alzheimer Disease Brains [J].
Benseny-Cases, Nuria ;
Klementieva, Oxana ;
Cotte, Marine ;
Ferrer, Isidre ;
Cladera, Josep .
ANALYTICAL CHEMISTRY, 2014, 86 (24) :12047-12054
[7]   Hormetics: Dietary Triggers of an Adaptive Stress Response [J].
Birringer, Marc .
PHARMACEUTICAL RESEARCH, 2011, 28 (11) :2680-2694
[8]   Photosensitizer effects on cancerous cells:: A combined study using synchrotron infrared and fluorescence microscopies [J].
Chio-Srichan, Sirinart ;
Refregiers, Matthieu ;
Jamme, Frederic ;
Kascakova, Slavka ;
Rouam, Valerie ;
Dumas, Paul .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2008, 1780 (05) :854-860
[9]   Isolation and characterization of human primary enterocytes from small intestine using a novel method [J].
Chougule, Priti ;
Herlenius, Gustaf ;
Hernandez, Nidia Maritza ;
Patil, Pradeep B. ;
Xu, Bo ;
Sumitran-Holgersson, Suchitra .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 2012, 47 (11) :1334-1343
[10]   Synchrotron FTIR micro-spectroscopy study of the rat hippocampal formation after pilocarpine-evoked seizures [J].
Chwiej, J. ;
Dulinska, J. ;
Janeczko, K. ;
Dumas, P. ;
Eichert, D. ;
Dudala, J. ;
Setkowicz, Z. .
JOURNAL OF CHEMICAL NEUROANATOMY, 2010, 40 (02) :140-147
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