Dissecting the human immune system with single cell RNA sequencing technology

被引：12

作者：

Xu, Gang ^{[1
,2
]}

Liu, Yang ^{[1
,2
]}

Li, Hanjie ^{[3
]}

Liu, Lei ^{[1
,2
]}

Zhang, Shuye ^{[4
,5
]}

Zhang, Zheng ^{[1
,2
,6
,7
]}

机构：

[1] Southern Univ Sci & Technol, Affiliated Hosp 2, Shenzhen Peoples Hosp 3, Inst Hepatol,Natl Clin Res Ctr Infect Dis, Shenzhen 518100, Guangdong, Peoples R China

[2] Shenzhen Third Peoples Hosp, Guangdong Key Lab Emerging Infect Dis, Shenzhen, Guangdong, Peoples R China

[3] Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen, Guangdong, Peoples R China

[4] Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai 201508, Peoples R China

[5] Fudan Univ, Inst Biomed Sci, Shanghai 201508, Peoples R China

[6] Guangzhou Med Univ, Affiliated Hosp 2, Sch Basic Med Sci, Key Lab Immunol,Sino French Hoffmann Inst, Guangzhou, Guangdong, Peoples R China

[7] Guangzhou Med Univ, Affiliated Hosp 2, Guangdong Prov Key Lab Allergy & Clin Immunol, Guangzhou, Guangdong, Peoples R China

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 2020年 / 107卷 / 04期

关键词：

cell type identification; human immune system; single-cell RNA sequencing; trajectory inference; DIFFERENTIAL EXPRESSION ANALYSIS; MASS CYTOMETRY; T-CELLS; SEQ; HETEROGENEITY; LANDSCAPE; QUANTIFICATION; RECONSTRUCTION; EXHAUSTION; PROGENITOR;

D O I：

10.1002/JLB.5MR1019-179R

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Single-cell RNA sequencing (scRNA-seq) is a powerful new technology allowing the analysis of transcriptomes from individual cell and is ideally suited to dissect immune cell heterogeneity. ScRNA-seq has already been applied to identify novel immune cell subsets, elaborate cellular differentiation trajectories, and elucidate immunopathogenic mechanisms. Here, we briefly discuss the recent progresses and challenges in the scRNA-seq technology including the workflow, recent applications in immunology, and potential hurdles that need to be overcome. This review will highlight how single cell technology promotes our understanding of human immunology.

引用

页码：613 / 623

页数：11

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