Higher Plant Calreticulins Have Acquired Specialized Functions in Arabidopsis

被引:69
作者
Christensen, Anna [1 ,2 ]
Svensson, Karin [3 ]
Thelin, Lisa [1 ]
Zhang, Wenjing [3 ]
Tintor, Nico [4 ]
Prins, Daniel [5 ]
Funke, Norma
Michalak, Marek [5 ]
Schulze-Lefert, Paul [4 ]
Saijo, Yusuke [4 ]
Sommarin, Marianne [1 ,2 ]
Widell, Susanne [3 ]
Persson, Staffan [6 ]
机构
[1] Lund Univ, Dept Biochem, Ctr Chem & Chem Engn, Lund, Sweden
[2] Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, S-90187 Umea, Sweden
[3] Lund Univ, Dept Cell & Organism Biol, Lund, Sweden
[4] Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, Cologne, Germany
[5] Univ Alberta, Dept Biochem, Edmonton, AB, Canada
[6] Max Planck Inst Mol Plant Physiol, Potsdam, Germany
基金
瑞典研究理事会;
关键词
ENDOPLASMIC-RETICULUM; QUALITY-CONTROL; EXPRESSION; PROTEIN; IDENTIFICATION; CALCIUM; GENE; CHAPERONE; RECEPTOR; CALNEXIN;
D O I
10.1371/journal.pone.0011342
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Calreticulin (CRT) is a ubiquitous ER protein involved in multiple cellular processes in animals, such as protein folding and calcium homeostasis. Like in animals, plants have evolved divergent CRTs, but their physiological functions are less understood. Arabidopsis contains three CRT proteins, where the two CRTs AtCRT1a and CRT1b represent one subgroup, and AtCRT3 a divergent member. Methodology/Principal Findings: Through expression of single Arabidopsis family members in CRT-deficient mouse fibroblasts we show that both subgroups have retained basic CRT functions, including ER Ca2+-holding potential and putative chaperone capabilities. However, other more general cellular defects due to the absence of CRT in the fibroblasts, such as cell adhesion deficiencies, were not fully restored. Furthermore, in planta expression, protein localization and mutant analyses revealed that the three Arabidopsis CRTs have acquired specialized functions. The AtCRT1a and CRT1b family members appear to be components of a general ER chaperone network. In contrast, and as recently shown, AtCRT3 is associated with immune responses, and is essential for responsiveness to the bacterial Pathogen-Associated Molecular Pattern (PAMP) elf18, derived from elongation factor (EF)-Tu. Whereas constitutively expressed AtCRT1a fully complemented Atcrt1b mutants, AtCRT3 did not. Conclusions/Significance: We conclude that the physiological functions of the two CRT subgroups in Arabidopsis have diverged, resulting in a role for AtCRT3 in PAMP associated responses, and possibly more general chaperone functions for AtCRT1a and CRT1b.
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页数:18
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