Ovomucoid partitioning in aqueous two-phase systems

被引：25
|
作者：
de Oliveira, Fabiola Cristina [1 ]
dos Reis Coimbra, Jane Selia [1 ]
Mendes da Silva, Luis Henrique [2 ]
Garcia Rojas, Edwin Elard [3 ]
Hespanhol da Silva, Maria do Carmo [2 ]
机构：
[1] Univ Fed Vicosa, Dept Food Technol, BR-36570000 Vicosa, MG, Brazil
[2] Univ Fed Vicosa, Dept Chem, BR-36570000 Vicosa, MG, Brazil
[3] Univ Fed Fluminense, Dept Agribusiness & Adm Engn, BR-27225250 Volta Redonda, RJ, Brazil
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2009年 / 47卷 / 1-3期
关键词：
Aqueous two-phase; Extraction; Bioseparation; Partitioning; Egg white; Ovomucoid; LIQUID-LIQUID; EGG-WHITE; POLY(ETHYLENE GLYCOL); POLYETHYLENE-GLYCOL; 3RD DOMAIN; PROTEINS; MODEL; PURIFICATION; EQUILIBRIUM; EXTRACTION;
D O I：
10.1016/j.bej.2009.07.002
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This study evaluated the partitioning of ovomucoid from egg white, in aqueous two-phase systems (ATPS) composed of PEG 1500 and inorganic salt (lithium sulfate, sodium sulfate, magnesium sulfate, sodium carbonate or sodium citrate) at 25 degrees C. The results showed a great effect of the electrolyte nature on the partition coefficient. The partition coefficient value ranges from 0.02 to 6.0. The highest partition coefficients were obtained from systems composed of sodium carbonate and the lowest in systems composed of magnesium sulfate. In the system containing magnesium sulfate, a recovery percentage greater than 90% was obtained. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：55 / 60
页数：6
相关论文
共 50 条
  • [1] Partitioning of streptokinase in aqueous two-phase systems
    Otto, A.
    Lorenz, G.
    Kopperschlager, G.
    Bioseparation, 5 (01): : 35 - 40
  • [2] Partitioning of caseinomacropeptide in aqueous two-phase systems
    da Silva, Cesar A. Sodre
    Coimbra, Jane S. R.
    Rojas, Edwin E. Garcia
    Minim, Luis A.
    da Silva, Luis Henrique Mendes
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 858 (1-2): : 205 - 210
  • [3] Partitioning of glycomacropeptide in aqueous two-phase systems
    da Silva, C. A. S.
    Coimbra, J. S. R.
    Rojas, E. E. G.
    Teixeira, J. A. C.
    PROCESS BIOCHEMISTRY, 2009, 44 (11) : 1213 - 1216
  • [4] Whey protein partitioning in aqueous two-phase systems
    Valle-Guadarrama, Salvador
    Dominguez-Puerto, Ricardo
    Guerra-Ramirez, Diana
    BIOTECNIA, 2021, 23 (03): : 117 - 124
  • [5] Hydrophobic partitioning of proteins in aqueous two-phase systems
    Hachem, F
    Andrews, BA
    Asenjo, JA
    ENZYME AND MICROBIAL TECHNOLOGY, 1996, 19 (07) : 507 - 517
  • [6] Cell partitioning in aqueous two-phase polymer systems
    Cabral, J. M. S.
    CELL SEPARATION: FUNDAMENTALS, ANALYTICAL AND PREPARATIVE METHODS, 2007, 106 : 151 - 171
  • [7] Driving forces for phase separation and partitioning in aqueous two-phase systems
    Johansson, HO
    Karlström, G
    Tjerneld, F
    Haynes, CA
    JOURNAL OF CHROMATOGRAPHY B, 1998, 711 (1-2): : 3 - 17
  • [8] Partitioning of Chromobacterium viscosum lipases in aqueous two-phase systems
    Queiroz, J
    Garcia, FAP
    Cabral, JMS
    BIOSEPARATION, 1995, 5 (05) : 307 - 311
  • [9] Partitioning and purification of monoclonal antibodies in aqueous two-phase systems
    Andrews, BA
    Nielsen, S
    Asenjo, JA
    BIOSEPARATION, 1996, 6 (05) : 303 - 313
  • [10] Metal affinity partitioning of hemoglobin in an aqueous two-phase systems
    Guinn, MR
    Todd, PW
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1996, 211 : 91 - BIOT
12345