Production of L-DOPA using Cu-alginate gel immobilized tyrosinase in a batch and packed bed reactor

被引：60

作者：

Ates, Selma ^{[1
]}

Cortenlioglu, Esra

Bayraktar, Emine

Mehmetoglu, Ulku

机构：

[1] Gazi Univ, Fac Arts & Sci, Dept Chem, TR-06500 Ankara, Turkey

[2] Ankara Univ, Fac Engn, Dept Chem Engn, TR-06100 Ankara, Turkey

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2007年 / 40卷 / 04期

关键词：

L-DOPA production; tyrosinase; immobilized enzymes; packed bed reactor; copper alginate gel;

D O I：

10.1016/j.enzmictec.2006.05.031

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, production of L-DOPA (L-3, 4-dihydroxy phenylalanine) was investigated by using tyrosinase enzyme in batch and packed bed reactors. Tyrosinase has been immobilized with method of entrapment in copper-alginate gels. L-DOPA concentration obtained from batch reactor for free and immobilized enzyme was 9.5 and 4.5 mg/L, respectively. L-DOPA concentration was obtained as 1.2 mg/L in the packed bed reactor. When air was introduced L-DOPA production increased 6.4 times in packed bed reactor and the productivity was calculated as 110mg/Lh. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：683 / 687

页数：5

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