Mutations in lysX as the new and reliable markers for tuberculosis Beijing and modern Beijing strains

被引：0
作者：
Zhao, Li-li [1 ]
Xiao, Tong-yang [1 ]
Sun, Qing [1 ,2 ]
Liu, Hai-can [1 ]
Zhao, Xiu-qin [1 ]
Jiang, Yi [1 ]
Li, Gui-lian [1 ]
Zeng, Chun-yan [3 ]
Wan, Kang-lin [1 ,2 ]
机构：
[1] Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Collaborat Innovat Ctr Diag & Treatment Infect Di, Natl Inst Communicable Dis Control & Prevent, Beijing 102206, Peoples R China
[2] Hunan Tradit Chinese Med Coll, Dept Med, Zhuzhou 412012, Peoples R China
[3] Hulunbeier Peoples Hosp, Hulunbeier 021000, Peoples R China
来源：
TUBERCULOSIS | 2016年 / 97卷
基金：
中国国家自然科学基金;
关键词：
Mycobacterium tuberculosis; Beijing genotype; Modern Beijing subtype; Marker; Genotype; Multiplex allele-specific PCR; MYCOBACTERIUM-TUBERCULOSIS; GENETIC DIVERSITY; MUTT GENES; FAMILY; GENOTYPE; LINEAGE; TRANSMISSION; SUBLINEAGE; ORIGIN; PCR;
D O I：
10.1016/j.tube.2015.12.006
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Genotyping results and DNA sequencing analysis of 235 Mycobacterium tuberculosis (M. tuberculosis) isolates from China indicated that mutations at codon 995 (Pro CCG to Pro CCA) and 701 (Ile ATT to Thr ACT) in lysX gene (Rv1640c), are specific markers for Beijing and modern Beijing strains, respectively. This observation was also confirmed by 24 genomes of M. tuberculosis strains from other countries. Moreover, a simple and fast multiplex allele-specific PCR (MAS-PCR) method for detecting mutations at codon 995 and 701 in lysX has been established and used to screen 235 DNA samples obtained from M. tuberculosis isolates. In all cases, Beijing and modern Beijing strains were identified correctly. (c) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：33 / 37
页数：5
相关论文
共 36 条
  • [1] Mycobacterium tuberculosis strains with the Beijing genotype demonstrate variability in virulence associated with transmission
    Aguilar L, D.
    Hanekom, M.
    Mata, D.
    Gey van Pittius, N. C.
    van Helden, P. D.
    Warren, R. M.
    Hernandez-Pando, R.
    [J]. TUBERCULOSIS, 2010, 90 (05) : 319 - 325
  • [2] I-Mutant2.0: predicting stability changes upon mutation from the protein sequence or structure
    Capriotti, E
    Fariselli, P
    Casadio, R
    [J]. NUCLEIC ACIDS RESEARCH, 2005, 33 : W306 - W310
  • [3] Evolution and transmission of drug-resistant tuberculosis in a Russian population
    Casali, Nicola
    Nikolayevskyy, Vladyslav
    Balabanova, Yanina
    Harris, Simon R.
    Ignatyeva, Olga
    Kontsevaya, Irina
    Corander, Jukka
    Bryant, Josephine
    Parkhill, Julian
    Nejentsev, Sergey
    Horstmann, Rolf D.
    Brown, Timothy
    Drobniewski, Francis
    [J]. NATURE GENETICS, 2014, 46 (03) : 279 - +
  • [4] Deletion-targeted multiplex PCR (DTM-PCR) for identification of Beijing/W genotypes of Mycobacterium tuberculosis
    Chen, Jing
    Tsolaki, Anthony G.
    Shen, Xin
    Jiang, Xi
    Mei, Jim
    Gao, Qian
    [J]. TUBERCULOSIS, 2007, 87 (05) : 446 - 449
  • [5] Progression to active tuberculosis, but not transmission, varies by Mycobacterium tuberculosis lineage in the Gambia
    de Jong, Bouke C.
    Hill, Philip C.
    Aiken, Alex
    Awine, Timothy
    Antonio, Martin
    Adetifa, Ifedayo M.
    Jackson-Sillah, Dolly J.
    Fox, Annette
    DeRiemer, Kathryn
    Gagneux, Sebastien
    Borgdorff, Martien W.
    McAdam, Keith P. W. J.
    Corrah, Tumani
    Small, Peter M.
    Adegbola, Richard A.
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2008, 198 (07) : 1037 - 1043
  • [6] Beijing/W genotype Mycobacterium tuberculosis and drug resistance
    Glynn, JR
    Kremer, K
    Borgdorff, MW
    Rodriguez, MP
    van Soolingen, D
    [J]. EMERGING INFECTIOUS DISEASES, 2006, 12 (05) : 736 - 743
  • [7] Genetic diversity of the Mycobacterium tuberculosis Beijing family in Brazil and Mozambique and relation with infectivity and induction of necrosis in THP-1 cells
    Gomes, Lia Lima
    Goncalves Vasconcellos, Sidra Ezidrio
    Gomes, Harrison Magdinier
    Elias, Atina Ribeiro
    Rocha, Adalgiza da Silva
    Ribeiro, Simone C. M.
    Panunto, Alessandra Costa
    Ferrazoli, Lucilaine
    da Silva Telles, Maria Alice
    Emanuel Ivens de, Araujo Marelo
    Kritski, Afranio Lineu
    Mokrousov, Igor
    Manicheva, Olga A.
    Lasunskaia, Elena
    Suffys, Philip Noel
    [J]. TUBERCULOSIS, 2015, 95 : S190 - S196
  • [8] A recently evolved sublineage of the Mycobacterium tuberculosis Beijing strain family is associated with an increased ability to spread and cause disease
    Hanekom, M.
    van der Spuy, G. D.
    Streicher, E.
    Ndabambi, S. L.
    McEvoy, C. R. E.
    Kidd, M.
    Beyers, N.
    Victor, T. C.
    van Helden, P. D.
    Warren, R. M.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (05) : 1483 - 1490
  • [9] Comparative Genomic Analysis of Mycobacterium tuberculosis Drug Resistant Strains from Russia
    Ilina, Elena N.
    Shitikov, Egor A.
    Ikryannikova, Larisa N.
    Alekseev, Dmitry G.
    Kamashev, Dmitri E.
    Malakhova, Maja V.
    Parfenova, Tatjana V.
    Afanas'ev, Maxim V.
    Ischenko, Dmitry S.
    Bazaleev, Nikolai A.
    Smirnova, Tatjana G.
    Larionova, Elena E.
    Chernousova, Larisa N.
    Beletsky, Alexey V.
    Mardanov, Andrei V.
    Ravin, Nikolai V.
    Skryabin, Konstantin G.
    Govorun, Vadim M.
    [J]. PLOS ONE, 2013, 8 (02):
  • [10] Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology
    Kamerbeek, J
    Schouls, L
    Kolk, A
    vanAgterveld, M
    vanSoolingen, D
    Kuijper, S
    Bunschoten, A
    Molhuizen, H
    Shaw, R
    Goyal, M
    vanEmbden, J
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (04) : 907 - 914
1234