Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer

被引:10
|
作者
Dudea-Simon, Marina [1 ]
Mihu, Dan [1 ]
Pop, Laura Ancuta [2 ]
Ciortea, Razvan [1 ]
Malutan, Andrei Mihai [1 ]
Diculescu, Doru [1 ]
Ciocan, Cristina Alexandra [2 ]
Cojocneanu, Roxana Maria [2 ]
Simon, Vasile [3 ]
Bucuri, Carmen [1 ]
Mocan-Hognogi, Radu [1 ]
Braicu, Cornelia [2 ]
Berindan-Neagoe, Ioana [2 ]
机构
[1] Iuliu Hatieganu Univ Med & Pharm, Domin Stanca Obstet & Gynecol Clin, Obstet & Gynecol Dept 2, Cluj Napoca 400012, Romania
[2] Iuliu Hatieganu Univ Med & Pharm, Res Ctr Funct Genom Biomed & Translat Med, 15 Victor Babes St, Cluj Napoca 400012, Romania
[3] Iuliu Hatieganu Univ Med & Pharm, Urol Dept, Cluj Napoca 400012, Romania
关键词
cervical cancer; cervical intraepithelial neoplasia; long noncoding RNAs; microRNA; HUMAN-PAPILLOMAVIRUS; PROLIFERATION; METASTASIS; BIOMARKERS; HPV;
D O I
10.3390/ijms23116054
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Cervical cancer is one of the most common malignancies in women in terms of prevalence and mortality. Cervical cancer has some particularities that distinguish it from any other oncologic pathology: first, it is completely preventable by prompt detection of its precursor, cervical intraepithelial neoplasia (CIN); second, the Human Papillomavirus (HPV) infection is a known etiological agent; third, the mean age at diagnosis is much lower than in other oncologic conditions, as a consequence of the sexually-transmitted HPV. Methods: We evaluated the expression level of several long noncoding RNAs and a microRNA in samples from 30 patients with CIN, 9 with cervical cancer and 38 normal samples using qRT-PCR technology. Results: We observed higher expression levels for MEG3, DAPK1, MLH1 and MALAT1 in CIN samples than in normal samples, whereas TIMP3 and SOX1 had lower expression levels. For cancer samples, DAPK1, MLH1 and MALAT1 had higher expression, and MEG3, TIMP3 and SOX1 had lower expression when compared to normal samples. In the case of CIN versus cancer samples, only MEG3 gene showed a statistically significant difference. The expression of miR-205-5p was lower in both CIN and cancer samples compared to normal samples. Conclusion: Decreased MEG3 expression could be considered an alarm signal in the transition from a premalignant cervical lesion to invasive cancer, while altered expression levels of TIMP3, SOX1, MLH1, MALAT1 and miR-205-5p could serve as early biomarkers in the diagnosis of premalignant cervical lesions. Future studies, including a larger number of patients with CIN, will be of particular importance in validating these observations.
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页数:15
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