Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer

被引:11
|
作者
Dudea-Simon, Marina [1 ]
Mihu, Dan [1 ]
Pop, Laura Ancuta [2 ]
Ciortea, Razvan [1 ]
Malutan, Andrei Mihai [1 ]
Diculescu, Doru [1 ]
Ciocan, Cristina Alexandra [2 ]
Cojocneanu, Roxana Maria [2 ]
Simon, Vasile [3 ]
Bucuri, Carmen [1 ]
Mocan-Hognogi, Radu [1 ]
Braicu, Cornelia [2 ]
Berindan-Neagoe, Ioana [2 ]
机构
[1] Iuliu Hatieganu Univ Med & Pharm, Domin Stanca Obstet & Gynecol Clin, Obstet & Gynecol Dept 2, Cluj Napoca 400012, Romania
[2] Iuliu Hatieganu Univ Med & Pharm, Res Ctr Funct Genom Biomed & Translat Med, 15 Victor Babes St, Cluj Napoca 400012, Romania
[3] Iuliu Hatieganu Univ Med & Pharm, Urol Dept, Cluj Napoca 400012, Romania
关键词
cervical cancer; cervical intraepithelial neoplasia; long noncoding RNAs; microRNA; HUMAN-PAPILLOMAVIRUS; PROLIFERATION; METASTASIS; BIOMARKERS; HPV;
D O I
10.3390/ijms23116054
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Cervical cancer is one of the most common malignancies in women in terms of prevalence and mortality. Cervical cancer has some particularities that distinguish it from any other oncologic pathology: first, it is completely preventable by prompt detection of its precursor, cervical intraepithelial neoplasia (CIN); second, the Human Papillomavirus (HPV) infection is a known etiological agent; third, the mean age at diagnosis is much lower than in other oncologic conditions, as a consequence of the sexually-transmitted HPV. Methods: We evaluated the expression level of several long noncoding RNAs and a microRNA in samples from 30 patients with CIN, 9 with cervical cancer and 38 normal samples using qRT-PCR technology. Results: We observed higher expression levels for MEG3, DAPK1, MLH1 and MALAT1 in CIN samples than in normal samples, whereas TIMP3 and SOX1 had lower expression levels. For cancer samples, DAPK1, MLH1 and MALAT1 had higher expression, and MEG3, TIMP3 and SOX1 had lower expression when compared to normal samples. In the case of CIN versus cancer samples, only MEG3 gene showed a statistically significant difference. The expression of miR-205-5p was lower in both CIN and cancer samples compared to normal samples. Conclusion: Decreased MEG3 expression could be considered an alarm signal in the transition from a premalignant cervical lesion to invasive cancer, while altered expression levels of TIMP3, SOX1, MLH1, MALAT1 and miR-205-5p could serve as early biomarkers in the diagnosis of premalignant cervical lesions. Future studies, including a larger number of patients with CIN, will be of particular importance in validating these observations.
引用
收藏
页数:15
相关论文
共 50 条
  • [1] Cervical intraepithelial neoplasia, cervical cancer, and HPV
    Holly, EA
    ANNUAL REVIEW OF PUBLIC HEALTH, 1996, 17 : 69 - 84
  • [2] Tissue expression of VEGF in cervical intraepithelial neoplasia and cervical cancer
    Mandic, Aljosa
    Knezevic, Slavica Usaj
    Ivkovic, Tatjana Kapicl
    JOURNAL OF BUON, 2014, 19 (04): : 958 - 964
  • [3] Oncogenic microRNA signature for early diagnosis of cervical intraepithelial neoplasia and cancer
    Liu, Stephanie S.
    Chan, Karen K. L.
    Chu, Daniel K. H.
    Wei, Tina N.
    Lau, Lesley S. K.
    Ngu, Siew F.
    Chu, Mandy M. Y.
    Tse, Ka Yu
    Ip, Philip P. C.
    Ng, Enders K. O.
    Cheung, Annie N. Y.
    Ngan, Hextan Y. S.
    MOLECULAR ONCOLOGY, 2018, 12 (12): : 2009 - 2022
  • [4] A circulating serum miRNA panel as early detection biomarkers of cervical intraepithelial neoplasia
    Xin, F.
    Liu, P.
    Ma, C-F.
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2016, 20 (23) : 4846 - 4851
  • [5] The Expression of Cyclooxygenase-2 in Cervical Intraepithelial Neoplasia and Cervical Cancer
    Shaik, Nuzhath
    Nerune, Savitri M.
    Patil, Vijayalaxmi S.
    Jawalkar, Sneha
    CUREUS JOURNAL OF MEDICAL SCIENCE, 2024, 16 (08)
  • [6] Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer
    Sato, Atsuki
    Ishiwata, Toshiyuki
    Matsuda, Yoko
    Yamamoto, Tetsushi
    Asakura, Hirobumi
    Takeshita, Toshiyuki
    Naito, Zenya
    INTERNATIONAL JOURNAL OF ONCOLOGY, 2012, 41 (02) : 441 - 448
  • [7] PCNA in Cervical Intraepithelial Neoplasia and Cervical Cancer: An Interaction Network Analysis of Differentially Expressed Genes
    Giannos, Panagiotis
    Kechagias, Konstantinos S.
    Bowden, Sarah
    Tabassum, Neha
    Paraskevaidi, Maria
    Kyrgiou, Maria
    FRONTIERS IN ONCOLOGY, 2021, 11
  • [8] Comparison of human papillomavirus genotype distributions in cervical intraepithelial neoplasia and cervical cancer
    Li, Xiaolin
    Wan, Xiaojie
    Zheng, Feiyun
    Zhu, Haiyan
    Zhu, Xuejie
    Yu, Jianqin
    BIOMEDICAL RESEARCH-INDIA, 2017, 28 (05): : 2284 - 2289
  • [9] Determination of malignant potential of cervical intraepithelial neoplasia
    Kudela, E.
    Holubekova, V.
    Farkasova, A.
    Danko, J.
    TUMOR BIOLOGY, 2016, 37 (02) : 1521 - 1525
  • [10] Cervical intraepithelial neoplasia
    Crum, CP
    McLachlin, CM
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1995, : 71 - 79