The impact of ExHp-CD (outer membrane vesicles) released from Helicobacter pylori SS1 on macrophage RAW 264.7 cells and their immunogenic potential

被引：19

作者：

Ahmed, Abeer Ahmed Qaed ^{[1
]}

Qi, Fuyu ^{[1
]}

Zheng, Ruizhu ^{[1
]}

Xiao, Lin ^{[2
]}

Abdalla, Ahmed M. E. ^{[3
]}

Mao, Lin ^{[2
]}

Bakadia, Bianza Moise ^{[1
]}

Liu, Li ^{[1
]}

Atta, Omar Mohammad ^{[1
]}

Li, Xiaohong ^{[1
]}

Shi, Zhijun ^{[1
]}

Yang, Guang ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Dept Biomed Engn, 1037 Luoyu Rd, Wuhan 430074, Peoples R China

[2] Sun Yat Sen Univ, Sch Biomed Engn, Guangzhou 510006, Peoples R China

[3] Univ Bahri, Coll Appl Sci, Dept Biochem, Khartoum 166011111, Sudan

来源：

LIFE SCIENCES | 2021年 / 279卷

基金：

中国国家自然科学基金;

关键词：

Helicobacter pylori; Outer membrane vesicles; ExHp-CD; Epimerase_2 domain-containing protein; Probable cytosol aminopeptidase; Probable malate; quinone oxidoreductase; GRAM-NEGATIVE BACTERIA; EXTRACELLULAR VESICLES; T-CELLS; EXOSOMES; PROTEIN; INFECTION; GASTRITIS; IMMUNIZATION; ACTIVATION; PLATFORM;

D O I：

10.1016/j.lfs.2021.119644

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Bacterial-derived extracellular vesicles could play a major role in attenuating and treating diseases. They play a major anti-infection role by modulating immune responses against pathogens and preventing infection by inhibiting pathogen localization and proliferation. In this study, outer membrane vesicles (ExHp-CD) released by Helicobacter pylori SS1 (H. pylori) and total antigens isolated from H. pylori SS1 (AgHp) were evaluated for their immunogenic potential and their effect on macrophage RAW 264.7 cells. Results demonstrated that both ExHpCD and AgHp induced T helper 2 (Th2) immune response, which was reported to be important in immune protection against H. pylori infections. Both ExHp-CD and AgHp produced high levels of IL-10 and IL-4, while no significant levels of IL-12 p70 or IFN-gamma were detected. However, ExHp-CD showed a better effect on macrophage RAW 264.7 cells compared to AgHp. Macrophage RAW 264.7 cells stimulated with 5, and 10 mu g/mL of ExHp-CD showed an increased ratio of CD206 (M2 phenotype marker) and a decreased ratio of CD86 (M1 phenotype marker). Moreover, results suggested that the immunogenic effect that ExHp-CD possesses was attributed to their cargo of Epimerase_2 domain-containing protein (Epi_2D), Probable malate:quinone oxidoreductase (Pro_mqo), and Probable cytosol aminopeptidase (Pro_ca). Results demonstrated that ExHp-CD possesses an immunological activity to induce Th2 immune response against H. pylori infection with results comparable to AgHp. However, ExHp-CD showed higher efficacy regarding safety, biocompatibility, lack of toxicity, and hemocompatibility. Thus, it could serve as an immunogenic candidate with more desired characteristics.

引用

页数：16