High-Resolution Cryo-Electron Microscopy Structures of Murine Norovirus 1 and Rabbit Hemorrhagic Disease Virus Reveal Marked Flexibility in the Receptor Binding Domains

被引：61

作者：

Katpally, Umesh ^{[1
]}

Voss, Neil R. ^{[2
]}

Cavazza, Tommaso ^{[1
]}

Taube, Stefan ^{[3
]}

Rubin, John R. ^{[5
]}

Young, Vivienne L. ^{[4
]}

Stuckey, Jeanne ^{[5
]}

Ward, Vernon K. ^{[4
]}

Virgin, Herbert W. ^{[6
]}

Wobus, Christiane E. ^{[3
]}

Smith, Thomas J. ^{[1
]}

机构：

[1] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA

[2] Scripps Res Inst, La Jolla, CA 92037 USA

[3] Univ Michigan, Sch Med, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA

[4] Univ Otago, Sch Med Sci, Dept Microbiol Immunol, Dunedin 9054, New Zealand

[5] Univ Michigan, Inst Life Sci, Ann Arbor, MI 48109 USA

[6] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 11期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1128/JVI.00314-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Our previous structural studies on intact, infectious murine norovirus 1 (MNV-1) virions demonstrated that the receptor binding protruding (P) domains are lifted off the inner shell of the virus. Here, the three-dimensional (3D) reconstructions of recombinant rabbit hemorrhagic disease virus (rRHDV) virus-like particles (VLPs) and intact MNV-1 were determined to similar to 8-angstrom resolution. rRHDV also has a raised P domain, and therefore, this conformation is independent of infectivity and genus. The atomic structure of the MNV-1 P domain was used to interpret the MNV-1 reconstruction. Connections between the P and shell domains and between the floating P domains were modeled. This observed P-domain flexibility likely facilitates virus-host receptor interactions.

引用

页码：5836 / 5841

页数：6

共 15 条

[1] Crystallization and preliminary crystallographic analysis of San Miguel sea lion virus: An animal calicivirus [J].

Chen, R ;

Neill, JD ;

Prasad, BVV .

JOURNAL OF STRUCTURAL BIOLOGY, 2003, 141 (02) :143-148

[2] X-ray structure of a native calicivirus: Structural insights into antigenic diversity and host specificity [J].

Chen, Rong ;

Neill, John D. ;

Estes, Mary K. ;

Prasad, B. V. Venkataram .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (21) :8048-8053

[3] STAT1-dependent innate immunity to a Norwalk-like virus [J].

Karst, SM ;

Wobus, CE ;

Lay, M ;

Davidson, J ;

Virgin, HW .

SCIENCE, 2003, 299 (5612) :1575-1578

[4] Pocket factors are unlikely to play a major role in the life cycle of human rhinovirus [J].

Katpally, Umesh ;

Smith, Thomas J. .

JOURNAL OF VIROLOGY, 2007, 81 (12) :6307-6315

[5] Appion: An integrated, database-driven pipeline to facilitate EM image processing [J].

Lander, Gabriel C. ;

Stagg, Scott M. ;

Voss, Neil R. ;

Cheng, Anchi ;

Fellmann, Denis ;

Pulokas, James ;

Yoshioka, Craig ;

Irving, Christopher ;

Mulder, Anke ;

Lau, Pick-Wei ;

Lyumkis, Dmitry ;

Potter, Clinton S. ;

Carragher, Bridget .

JOURNAL OF STRUCTURAL BIOLOGY, 2009, 166 (01) :95-102

[6] EMAN: Semiautomated software for high-resolution single-particle reconstructions [J].

Ludtke, SJ ;

Baldwin, PR ;

Chiu, W .

JOURNAL OF STRUCTURAL BIOLOGY, 1999, 128 (01) :82-97

[7] ACE: Automated CTF estimation [J].

Mallick, SP ;

Carragher, B ;

Potter, CS ;

Kriegman, DJ .

ULTRAMICROSCOPY, 2005, 104 (01) :8-29

[8] Versatile RHDV virus-like particles: Incorporation of antigens by genetic modification and chemical conjugation [J].

Peacey, Matthew ;

Wilson, Sarah ;

Baird, Margaret A. ;

Ward, Vernon K. .

BIOTECHNOLOGY AND BIOENGINEERING, 2007, 98 (05) :968-977

[9] X-ray crystallographic structure of the Norwalk virus capsid [J].

Prasad, BVV ;

Hardy, ME ;

Dokland, T ;

Bella, J ;

Rossmann, MG ;

Estes, MK .

SCIENCE, 1999, 286 (5438) :287-290

[10] 3-DIMENSIONAL STRUCTURE OF CALICIVIRUS [J].

PRASAD, BVV ;

MATSON, DO ;

SMITH, AW .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 240 (03) :256-264

← 1 2 →