Investigation of stratum corneum lipid model membranes with free fatty acid composition by neutron diffraction

被引：16

作者：

Ryabova, Natalia Yu. ^{[1
,2
]}

Kiselev, M. A. ^{[1
]}

Dante, S. ^{[3
]}

Hauss, T. ^{[3
,4
]}

Balagurov, A. M. ^{[1
]}

机构：

[1] Joint Inst Nucl Res, Frank Lab Neutron Phys, Dubna 141980, Moscow Region, Russia

[2] Moscow MV Lomonosov State Univ, Skobeltsyn Inst Nucl Phys, Moscow, Russia

[3] Helmholtz Zentrum Berlin Mat & Energie, Berlin, Germany

[4] Tech Univ Darmstadt, Inst Biochem, Darmstadt, Germany

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2010年 / 39卷 / 08期

关键词：

Neutron diffraction; Stratum corneum; Model lipid membrane; Hydration; X-RAY-DIFFRACTION; CHOLESTEROL-SULFATE; ORGANIZATION; DISORDERS; HYDRATION; BILAYERS; LAMELLAE; WATER;

D O I：

10.1007/s00249-009-0569-z

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The outermost epidermal layer, the stratum corneum (SC), is the main skin barrier. Studies of SC model systems enable characterization of the influence of individual lipids on the organization of the SC lipid matrix, which is the main pathway of water through the skin. This work presents a neutron diffraction study of the SC model membranes based on short-chain ceramide 6 with nearly realistic composition of free fatty acids (FFA) at physiological temperature of the SC. The influence of FFA and the effect of cholesterol-cholesterol sulfate substitution on the structure and hydration of the SC model membranes are described. The structure of the SC membrane with FFA is close to the structure of the earlier studied SC membrane based on short-chain palmitic acid (PA) and does not vary significantly under changes of the ratio of the main membrane components. FFA accelerates membrane swelling at the same low level of hydration of both PA- and FFA-containing membranes. The substitution of cholesterol sulfate by cholesterol in the membrane composition decreases membrane swelling and leads to phase separation in the model system.

引用

页码：1167 / 1176

页数：10

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