One-step preparation of N-doped grapheme quantum dots with high quantum yield for bioimaging and highly sensitive electrochemical detection of isoniazid

被引:9
作者
Sivaselvam, S. [1 ]
Viswanathan, C. [1 ]
Ponpandian, N. [1 ]
机构
[1] Bharathiar Univ, Dept Nanosci & Technol, Coimbatore 641046, Tamil Nadu, India
来源
BIOMATERIALS ADVANCES | 2022年 / 135卷
关键词
Nitrogen-doped graphene quantum dots; Biocompatibility; C; elegans; Bioimaging; Electrocatalyst; Isoniazid (INZ); CARBON; SENSOR; NANOCOMPOSITE; CYTOTOXICITY; RIFAMPICIN; CATALYST; OXIDE;
D O I
10.1016/j.bioadv.2022.212731
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Conventional techniques for synthesizing GQDs have a poor quantum yield (QY) that restricts their biological applications. Herein, we present a rapid, cost-effective and high quantum yield synthesis of nitrogen-doped graphene quantum dots (N-GQDs) through a scientific microwave reactor. The reaction parameters like microwave irradiation time, temperature, precursor concentration and pressure were optimized for achieving high quantum yield. The prepared N-GQDs exhibit bright blue fluorescence and excitation independent emission property with a quantum yield of 42.81%. In-vivo investigations on C. elegans revealed that the as-prepared N-GQDs are exceptionally biocompatible and maintain the normal physiological functioning of the primary and secondary targeted organs in nematodes. The synergetic effect of intestinal barrier and defecation behavior mitigates N-GQDs translocation into reproductive organs of nematode. In addition, the N-GQDs modified GCE was tested for electrochemical sensing characteristics towards the anti-tuberculosis drug isoniazid (INZ). The N-GQDs showed appreciable electrocatalytic performance towards INZ with high sensitivity (3.76 mu A mu M-1 cm(-1)). The differential pulse voltammetry (DPV) analysis of N-GQDs exhibit a lower detection limit of 10.91 nM for INZ. The N-GQDs modified sensor exhibits good reproducibility, excellent anti-interference ability and excellent analytical performance for INZ in real samples like human blood serum and urine samples.
引用
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页数:11
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