Virulence Factor Profiles of Escherichia coli Isolated from Pork and Chicken Meats Obtained from Retail Markets

被引：4
作者：
Choi, SunKeum
Lee, Min Hwa
Lee, Bog-Hieu
Jung, Ji-Youn [2 ]
Choi, Changsun [1 ]
机构：
[1] Chung Ang Univ, Coll Human Ecol, Dept Food & Nutr, Ansung 456756, South Korea
[2] Kongju Natl Univ, Dept Compan & Lab Anim Sci, Yesan 340702, South Korea
来源：
KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES | 2010年 / 30卷 / 01期
关键词：
Escherichia coli; astA; prevalence; pork; chicken; HEAT-STABLE ENTEROTOXIN-1; PERSISTENT DIARRHEA; CODING GENE; PREVALENCE; SEQUENCES; CALVES; PCR;
D O I：
10.5851/kosfa.2010.30.1.148
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The aim of this study was to investigate the virulence factor profiles of Escherichia coli strains isolated from pork and chicken meats purchased from retail markets in Korea. From 943 pork and 142 chicken meats, 217 isolates of E. coli were cultured. The presence of 11 virulence factors (elt, estI, estII, astA, stx, cdt, cnf, agg, inve, eae, afa) were detected by polymerase chain reaction. Forty one (18.9%) of 217 E. coli isolates carried at least one virulence factor. Among 175 E. coli isolates from pork, the detection rate of astA, elt, eae, estII, estI, afa, and cnf were 6.9%, 4.6%, 4.6%, 4.0%, 2.3%, 1.1%, and 0.6%, respectively. However, stx, agg, and cdt were not detected in our isolates. Therefore, we conclude that astA is the most prevalent virulence factor in E. coli isolates contaminated in pork and chicken meats in Korea.
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收藏
页码：148 / 153
页数：6
相关论文
共 26 条
[1]   ENTEROAGGREGATIVE ESCHERICHIA-COLI ASSOCIATED WITH PERSISTENT DIARRHEA IN A COHORT OF RURAL CHILDREN IN INDIA [J].
BHAN, MK ;
RAJ, P ;
LEVINE, MM ;
KAPER, JB ;
BHANDARI, N ;
SRIVASTAVA, R ;
KUMAR, R ;
SAZAWAL, S .
JOURNAL OF INFECTIOUS DISEASES, 1989, 159 (06) :1061-1064
[2]   ENTEROAGGREGATIVE ESCHERICHIA-COLI AND SALMONELLA ASSOCIATED WITH NONDYSENTERIC PERSISTENT DIARRHEA [J].
BHAN, MK ;
KHOSHOO, V ;
SOMMERFELT, H ;
RAJ, P ;
SAZAWAL, S ;
SRIVASTAVA, R .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1989, 8 (08) :499-502
[3]   Prevalence of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 gene and its relationship with fimbrial and enterotoxin genes in E-coli isolated from diarrheic piglets [J].
Choi, C ;
Kwon, D ;
Chae, C .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2001, 13 (01) :26-29
[4]  
De Rycke J, 1999, VET RES, V30, P221
[5]   Multiplex real-time PCR for exhaustive detection of diarrhoeagenic Escherichia coli [J].
Hidaka, A. ;
Hokyo, T. ;
Arikawa, K. ;
Fujihara, S. ;
Ogasawara, J. ;
Hase, A. ;
Hara-Kudo, Y. ;
Nishikawa, Y. .
JOURNAL OF APPLIED MICROBIOLOGY, 2009, 106 (02) :410-420
[6]  
Ishiguro F, 2005, JPN J INFECT DIS, V58, P119
[7]  
Itoh F., 1992, JPN J CLIN MED, V50, P343
[8]   Characteristics of cytotoxic necrotizing factor and cytolethal distending toxin producing Escherichia coli strains isolated from meat samples in Northern Ireland [J].
Kadhum, HJ ;
Ball, HJ ;
Oswald, E ;
Rowe, MT .
FOOD MICROBIOLOGY, 2006, 23 (05) :491-497
[9]  
Kobayashi Kazuhiro, 2002, Kansenshogaku Zasshi, V76, P911
[10]   Characterization of AfaE adhesins produced by extraintestinal and intestinal human Escherichia coli isolates:: PCR assays for detection of afa adhesins that do or do not recognize Dr blood group antigens [J].
Le Bouguénec, C ;
Lalioui, L ;
Du Merle, L ;
Jouve, M ;
Courcoux, P ;
Bouzari, S ;
Selvarangan, R ;
Nowicki, BJ ;
Germani, Y ;
Andremont, A ;
Gounon, P ;
Garcia, MI .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (05) :1738-1745
123