Preparation of BSA Molecule Surface-imprinted Material and Studies on its Macromolecule Recognition Characters

A bovine serum albumin (BAS) molecule surface-imprinted material with high performance was prepared through molecular design and process planning The hydroxyl groups on crosslinked polyvinyl alcohol (CPVA) microspheres were first made to be esterified with methyl acryloyl chloride (MAC) as reagent, and a great deal of polymerizable double bonds were introduced onto the surfaces of the microspheres, forming modified microspheres MA-CPVA. Subsequently, with MA-CPVA as matrix and with cationic acryloxyethyltrimethyl ammonium chloride (DAC) as functional monomer as well as with N,N'-methylene bisacrylamide (MBA) as crosslinker, in a aqueous solution,BAS surface-imprinted material MIP-PDAC/CPVA was smoothly prepared. In this imprinting process, the strong electrostatic interaction between the host-guest molecules was the foundation, and the graft-polymerization of DAC and the imprinting of BAS were carried out synchronously. The product microspheres MIP-PDAC/CPVA were characterized by FTIR and SEM. On this basis, the macromolecule recognition character of MIP-PDAC/CPVA microspheres for BAS was examined in depth. The experimental results show that MIP-PDAC/CPVA microspheres possess excellent binding affinity and very high recognition selectivity. The binding capacity of BAS on MIP-PDAC/CPVA microspheres reaches up to 108 mg/g, and the coefficient of selectivity of MIP-PDAC/CPVA towards BAS relative to the constrast protein bovine hemoglobin (BHb) actually gets up to 60.2.