Comparison of a novel high-throughput screening system with the Bottle assay for evaluating insecticide toxicity

被引：1

作者：

Dusfour, Isabelle ^{[1
]}

Achee, Nicole L. ^{[1
]}

Sardelis, Michael R. ^{[1
]}

Chareonviriyaphap, Theeraphap ^{[2
]}

Grieco, John P. ^{[1
]}

机构：

[1] Uniformed Serv Univ Hlth Sci, Dept Prevent Med & Biometr, Bethesda, MD 20814 USA

[2] Kasetsart Univ, Fac Agr, Dept Entomol, Bangkok 10900, Thailand

来源：

JOURNAL OF PESTICIDE SCIENCE | 2009年 / 34卷 / 04期

基金：

美国国家卫生研究院;

关键词：

Bottle assay; HTS; insecticide toxicity; mosquitoes; resistance;

D O I：

10.1584/jpestics.G09-22

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Insecticide toxicity is commonly evaluated for disease vectors by either the WHO test or Bottle assay. More recently, a high-throughput screening (HTS) system was developed for testing insecticide effects on mosquito behavior and mortality. We compared HTS with the Bottle assay to evaluate the toxicity of insecticides in a population of Aedes aegypti from Thailand. Both the HTS and Bottle assay system were determined to be equivalent. The two systems mainly differed (1) in reaction time, with mosquitoes reacting faster in the Bottle assay than HTS, (2) in knockdown and mortality at low doses. This information will guide the testing protocol for evaluating chemical effects on behavioral responses in various vector populations. (C)Pesticide Science Society of Japan

引用

页码：283 / 286

页数：4

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