Probing protein-protein interactions in real time

被引：213

作者：

Viani, MB ^{[1
]}

Pietrasanta, LI ^{[1
]}

Thompson, JB ^{[1
]}

Chand, A ^{[1
]}

Gebeshuber, IC ^{[1
]}

Kindt, JH ^{[1
]}

Richter, M ^{[1
]}

Hansma, HG ^{[1
]}

Hansma, PK ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

来源：

NATURE STRUCTURAL BIOLOGY | 2000年 / 7卷 / 08期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1038/77936

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used a prototype small cantilever atomic force microscope to observe, in real time, the interactions between individual protein molecules. In particular, we have observed individual molecules of the chaperonin protein GroES binding to and then dissociating from individual GroEL proteins, which were immobilized on a mica support. This work suggests that the small cantilever atomic force microscope is a useful tool for studying protein dynamics at the single molecule level.

引用

页码：644 / 647

页数：4

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