Characterization of Salmonella enterica serovar Typhimurium DT104 invasion in an epithelial cell line (IPEC J2) from porcine small intestine

被引：24
作者：
Brown, David R. [1 ]
Price, Lisa D. [1 ]
机构：
[1] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA
来源：
VETERINARY MICROBIOLOGY | 2007年 / 120卷 / 3-4期
关键词：
actin; intestinal epithelium; time course; neural Wiskott-Aldrich syndrome protein; Rho GTPase;
D O I：
10.1016/j.vetmic.2006.11.001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120 min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Racl GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Racl GTPase-independent changes in epithelial actin assembly. (c) 2006 Elsevier B.V. All rights reserved.
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页码：328 / 333
页数：6
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