Hyaluronic acid inhibits apoptosis in granulosa cells via CD44

被引：50
|
作者：
Kaneko, T [1 ]
Saito, H [1 ]
Toya, M [1 ]
Satio, T [1 ]
Nakahara, K [1 ]
Hiroi, M [1 ]
机构：
[1] Yamagata Univ, Sch Med, Dept Obstet & Gynecol, Yamagata 9909585, Japan
来源：
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2000年 / 17卷 / 03期
关键词：
apoptosis; CD44; human; hyaluronic acid; granulosa cells;
D O I：
10.1023/A:1009470206468
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Purpose: This study was designed to examine whether hyaluronic acid (HA) inhibits apoptosis in cumulus and mural granulosa cells and to examine whether this effect of HA was mediated through CD44. Methods: Mural and cumulus granulosa cells were obtained from in vitro fertilization patients. The cells were cultured with various concentrations of HA or HA pills various concentrations of anti-CD44 antibody without serum supplement. After 24 hr of culture, the cells were fixed and stained with Hoechst 33258. One thousand granulosa cells of each conditions were observed by fluorescence microscopy. Results: HA inhibited apoptosis in both kinds of granulosa cells, and anti-CD44 antibody prevented this effect of HA. Conclusions: The incidence of apoptotic granulosa cells with fragmented condensed nuclei was reduced by HA via CD44.
引用
收藏
页码：162 / 167
页数：6
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