Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD-PCR analysis

被引：42
作者：
Haryani, Y. [1 ]
Noorzaleha, A. S.
Fatimah, A. B.
Noorjahan, B. A.
Patrick, G. B.
Shamsinar, A. T.
Laila, R. A. S.
Son, R.
机构：
[1] Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Sci, Natl Ctr Food Safety Res, Serdang 43400, Selangor Darul, Malaysia
[2] Univ Riau, Fac Math & Sci, Dept Chem, Riau, Sumatera, Indonesia
[3] Dept Chem Malaysia, Petaling Jaya 46661, Selangor Darul, Malaysia
[4] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Cell & Mol Biol, Serdang 43400, Malaysia
[5] Natl Publ Hlth Lab, Sungai Buloh 47000, Selangor Darul, Malaysia
[6] Fed Govt Adm Ctr, Minist Hlth Malaysia, Food Safety & Qual Div, Putrajaya 62590, Malaysia
来源：
FOOD CONTROL | 2007年 / 18卷 / 07期
关键词：
street foods; Klebsiella pneumonia; plasmid; antibiotic resistance; RAPD; PCR;
D O I：
10.1016/j.foodcont.2006.04.009
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A total of 78 samples of street foods from different states in Malaysia were examined for the presence of Klebsiella pneumonia. K pneumonia contamination was recorded in 32% of the samples examined. The frequency of the K pneumonia isolates containing plasmids was shown to be 48%. Susceptibility testing showed that all isolates were highly resistant to ampicillin (100%), erythromycin (100%), rifampicin (100%), streptomycin (96%) and sulfamethoxazole (80%), but susceptible to chloramphenicol. RAPID proved to be the most effective technique in discriminating the K pneumonia isolates since dendrogram constructed using the combination of 4 primers could differentiate 25 isolates of K. pneumonia to 25 strains. This is the first report that revealed the occurrence of K. pneumonia in street foods sold in Malaysia. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：847 / 853
页数：7
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