Nascent Lep inserts into the Escherichia coli inner membrane in the vicinity of YidC, SecY and SecA

Targeting and assembly of the Escherichia coli inner membrane protein leader peptidase (Lep) was studied using a homologous in vitro targeting/translocation assay. Assembly of full-length Lep was efficient in the co-translational presence of membrane vesicles and hardly occurred when membranes were added post-translationally. This is consistent with the signal recognition particle-dependent targeting of Lep, Crosslinking experiments showed that the hydrophilic region Fl of nascent membrane-inserted Lep 100-mer was in the vicinity of SecA and SecY, whereas the first transmembrane domain HI was in the vicinity of YidC, These results suggested that YidC, together with the Sec translocase, functions in the assembly of Lep, YidC might be a more generic component in the assembly of inner membrane proteins. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.