DNA and RNA extraction from low amount of blood volume

被引：4
作者：
Farivar, Shirin [1 ]
Ghazimoradi, Mohammad H. [1 ]
机构：
[1] Shahid Beheshti Univ, Fac Life Sci & Biotechnol, Dept Cell & Mol Biol, Genet Stem Cells, Tehran 1983963113, Iran
来源：
FORENSIC SCIENCE INTERNATIONAL | 2019年 / 303卷
关键词：
DNA extraction; RNA extraction; TRI reagent; Blood samples; Sample volume; Yield;
D O I：
10.1016/j.forsciint.2019.109931
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
Clinical and research-based tests in molecular biology require a substantial amount of DNA and RNA, unfortunately, a considerable number of cells is needed for this amount of sample. Blood is one of the best and easiest source of cells, but is not used due to its invasive drawing methods and the needed volume. Another considerable point is the low amount of samples detected in crime scenes. TRI reagent is one of the most available methods for DNA, RNA and protein extraction. However, based on unsuccessful results, this method has not been widely used on blood samples. In this study, for the first time, the use of TRI reagent on micro scale blood volume was reported, resulting in high yield of DNA and RNA with great quality. (C) 2019 Published by Elsevier B.V.
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收藏
页数：4
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