Our recent progress in epigenetic research using the model ciliate, Tetrahymena thermophila

被引：32
作者：
Cheng, Ting [1 ,2 ,5 ]
Wang, Yuanyuan [1 ,2 ,5 ]
Huang, Jie [1 ,2 ,5 ]
Chen, Xiao [4 ]
Zhao, Xiaolu [3 ]
Gao, Shan [1 ,2 ,5 ]
Song, Weibo [1 ,2 ,5 ]
机构：
[1] Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266003, Peoples R China
[3] Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USA
[4] Columbia Univ, Dept Genet & Dev, Med Ctr, New York, NY 10032 USA
[5] Ocean Univ China, Coll Marine Life Sci, MOE Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China
来源：
MARINE LIFE SCIENCE & TECHNOLOGY | 2019年 / 1卷 / 01期
关键词：
Epigenetics; Tetrahymena; N-6-methyladenine (6mA); Nucleosome; Transposable elements; Histone methyltransferase TXR1; TRANSCRIPTION-REPLICATION CONFLICTS; N-6-METHYLADENINE DNA MODIFICATION; SET-DOMAIN PROTEINS; NUCLEOSOME DISTRIBUTION; UNICELLULAR EUKARYOTE; TRANSPOSABLE ELEMENTS; GENOME REARRANGEMENT; HISTONE METHYLATION; CHROMATIN; FAMILY;
D O I：
10.1007/s42995-019-00015-0
中图分类号：
Q17 [水生生物学];
学科分类号：
071004 ;
摘要：
Epigenetic research focuses on heritable changes beyond the DNA sequence, which has led to a revolution in biological studies and benefits in many other fields. The well-known model ciliate, Tetrahymena thermophila offers a unique system for epigenetic studies due to its nuclear dimorphism and special mode of sexual reproduction (conjugation), as well as abundant genomic resources and genetic tools. In this paper, we summarize recent progress made by our research team and collaborators in understanding epigenetic mechanisms using Tetrahymena. This includes: (1) providing the first genome-wide base pair-resolution map of DNA N-6-methyladenine (6mA) and revealed it as an integral part of the chromatin landscape; (2) dissecting the relative contribution of cis- and trans- elements to nucleosome distribution by exploring the unique nuclear dimorphism of Tetrahymena; (3) demonstrating the epigenetic controls of RNAi-dependent Polycomb repression pathways on transposable elements, and (4) identifying a new histone monomethyltransferase, TXR1 (Tetrahymena Trithorax 1), that facilitates replication elongation through its substrate histone H3 lysine 27 monomethylation (H3K27me1).
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页码：4 / 14
页数：11
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