Enzymatic Degradation of Gracilariopsis lemaneiformis Polysaccharide and the Antioxidant Activity of Its Degradation Products

被引:22
作者
Fang, Tian [1 ]
Zhang, Xiaoqian [1 ]
Hu, Shanshan [1 ]
Yu, Yanyan [1 ]
Sun, Xue [1 ]
Xu, Nianjun [1 ]
机构
[1] Ningbo Univ, Sch Marine Sci, Key Lab Marine Biotechnol Zhejiang Prov, Ningbo 315211, Peoples R China
基金
国家重点研发计划;
关键词
Gracilariopsis lemaneiformis; polysaccharides; enzymatic degradation; antioxidant activity; oxidative damage; INDUCED OXIDATIVE STRESS; SULFATED POLYSACCHARIDE; IN-VITRO; STRUCTURAL-CHARACTERIZATION; ASSISTED EXTRACTION; ANTITUMOR-ACTIVITY; PURIFICATION; CYTOTOXICITY; OPTIMIZATION; YEZOENSIS;
D O I
10.3390/md19050270
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Gracilariopsis lemaneiformis polysaccharides (GLP) were degraded using pectinase, glucoamylase, cellulase, xylanase, and beta-dextranase into low-molecular-weight polysaccharides, namely, GPP, GGP, GCP, GXP, and GDP, respectively, and their antioxidant capacities were investigated. The degraded GLP showed higher antioxidant activities than natural GLP, and GDP exhibited the highest antioxidant activity. After the optimization of degradation conditions through single-factor and orthogonal optimization experiments, four polysaccharide fractions (GDP1, GDP2, GDP3, and GDP4) with high antioxidant abilities (hydroxyl radical scavenging activity, DPPH radical scavenging activity, reduction capacity, and total antioxidant capacity) were obtained. Their cytoprotective activities against H2O2-induced oxidative damage in human fetal lung fibroblast 1 (HFL1) cells were examined. Results suggested that GDP pretreatment can significantly improve cell viability, reduce reactive oxygen species and malonaldehyde levels, improve antioxidant enzyme activity and mitochondria membrane potential, and alleviate oxidative damage in HFL1 cells. Thus, the enzyme degradation of GLP with beta-dextranase can significantly improve its antioxidant activity, and GDP might be a suitable source of natural antioxidants.
引用
收藏
页数:17
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