Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments

被引：29

作者：

Kobayashi, AK ^{[1
]}

Bespalhok, JC ^{[1
]}

Pereira, LFP ^{[1
]}

Vieira, LGE ^{[1
]}

机构：

[1] Inst Agron Parana, Lab Biotecnol Vegetal, BR-86001970 Londrina, Parana, Brazil

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2003年 / 74卷 / 01期

关键词：

adventitious buds; Citrus sinensis; mature tissue; sweet orange;

D O I：

10.1023/A:1023375526781

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An efficient system for in vitro plant regeneration from thin transversal stem sections explants (1-2 mm) using mature tissues of sweet orange cv. Pera was developed. Explants were cultured in different media to evaluate the frequency of regeneration and size of buds. A high percentage of explants (54% with 3.1 buds/explant) producing large buds (1-4 mm) was observed when the explants were cultivated for 2 weeks on Murashige and Skoog medium and then transferred to Woody Plant medium (WPM). Both media were supplemented with 1.8 muM 6-benzylaminopurine and 0.7 muM gibberellic acid. Adventitious buds were regenerated into whole plants by in vitro shoot-tip grafting. Regenerated plants started to flower after 12 months in the greenhouse, confirming their mature nature.

引用

页码：99 / 102

页数：4

共 10 条

[1]

BESPALHOK FJC, 2001, CROP BREED APPL BIOT, V1, P27

[2]

BESPALHOK FJC, 1992, BRAZILIAN J PLANT PH, V4, P59

[3] Agrobacterium-mediated transformation of the commercially important citrus cultivar Washington navel orange [J].

Bond, JE ;

Roose, ML .

PLANT CELL REPORTS, 1998, 18 (3-4) :229-234

[4] Genetic transformation and regeneration of mature tissues of woody fruit plants bypassing the juvenile stage [J].

Cervera M. ;

Juárez J. ;

Navarro A. ;

Pina J.A. ;

Durán-Vila N. ;

Navarro L. ;

Peña L. .

Transgenic Research, 1998, 7 (1) :51-59

[5] Green fluorescent protein as a screenable marker to increase the efficiency of generating transgenic woody fruit plants [J].

Ghorbel, R ;

Juárez, J ;

Navarro, L ;

Peña, L .

THEORETICAL AND APPLIED GENETICS, 1999, 99 (1-2) :350-358

[6] Factors affecting Agrobacterium-mediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of citrus tristeza virus [J].

GutierrezE, MA ;

Luth, D ;

Moore, GA .

PLANT CELL REPORTS, 1997, 16 (11) :745-753

[7]

Lloyd G., 1980, P INT PLANT PROP SOC, V30, P421

[8] AGROBACTERIUM-MEDIATED TRANSFORMATION OF CITRUS STEM SEGMENTS AND REGENERATION OF TRANSGENIC PLANTS [J].

MOORE, GA ;

JACONO, CC ;

NEIDIGH, JL ;

LAWRENCE, SD ;

CLINE, K .

PLANT CELL REPORTS, 1992, 11 (5-6) :238-242

[9] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].

MURASHIGE, T ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497

[10]

Navarro L., 1992, BIOTECHNOLOGY AGR FO, V18, P328

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