Embolization of a common carotid aneurysm with rhVEGF coupled to a pH-responsive chitosan in a rat model Laboratory investigation

被引:6
作者
Pan, Hui
Zimmerman, Thomas [2 ]
Zakhaleva, Julia [3 ]
Abrahams, John M. [4 ]
Jiang, Hongliang [5 ]
Chen, Weiliam [1 ]
机构
[1] SUNY Stony Brook, Hlth Sci Ctr, Dept Biomed Engn, Stony Brook, NY 11794 USA
[2] SUNY Stony Brook, Div Lab Anim Res, Stony Brook, NY 11794 USA
[3] SUNY Stony Brook, Dept Surg, Stony Brook, NY 11794 USA
[4] New York Med Coll, Dept Neurosurg, Valhalla, NY USA
[5] Zhejiang Univ, Dept Polymer Sci & Engn, Hangzhou 310003, Zhejiang, Peoples R China
基金
美国国家卫生研究院;
关键词
intracranial aneurysm; embolization; vascular endothelial growth factor; FIBROBLAST-GROWTH-FACTOR; INTRACRANIAL ANEURYSMS; IN-VITRO; RELEASE; COILS; VIVO;
D O I
10.3171/2009.1.JNS08411
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Object. Treatment of cerebral aneurysms by endovascular deployment of liquid embolic agents has been proposed as an alternative strategy to conventional coiling, and new materials are being developed for embolization. In this study, the authors used a single-injection, biocompatible, biodegradable and pH-responsive acrylated chitosan (aCHN) with conjugated vascular endothelial growth factor (rhVEGF) in a rat aneurysm model. Methods. The efficacy of the aCHN formulation with rhVEGF was tested using a common carotid artery occlusion model in rats, and the extent of embolization was evaluated using quantitative, qualitative, and histopathological techniques after 14 days of implantation. Results. The mean occlusion was significantly greater for the rhVEGF/aCHN-treated group (96.8 +/- 3.0%) than for the group receiving aCHN (74.7 +/- 5.6%) (p < 0.01). Through qualitative evaluation, intimal and medial proliferation were significantly greater with rhVEGF/aCHN than with aCHN and controls (p < 0.001). Degradation of the aCHN filler was monitored in concert with the production of extracellular matrix components. Macrophages migrated in and proliferated inside the occluded carotid artery lumens were identified by histological and immunostainings. Results showed resorption of chitosan with concurrent development of collagen and elastin into the vessel lumen, suggesting clot maturation into fibrosis. Conclusions. Chitosan with a bioactive agent such as rhVEGF showed excellent results in occluding aneurysms in a rat model. (DOI: 10.3171/2009.1.JNS08411)
引用
收藏
页码:658 / 665
页数:8
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