Analysis of genomic alterations in benign, atypical, and anaplastic meningiomas:: Toward a genetic model of meningioma progression

Nineteen benign [World Health Organization (WHO) grade I; MI], 21 atypical (WHO grade II; MH), and 19 anaplastic (WHO grade III; MIII) sporadic meningiomas were screened for chromosomal imbalances by comparative genomic hybridization (CGH), These data were supplemented by moleo ular genetic analyses of selected chromosomal regions and genes, With increasing malignancy grade, a marked accumulation of genomic aberrations was observed; i.e., the numbers (mean +/- SEM) of total alterations detected per tumor were 2.9 +/- 0.7 for MI, 9.2 +/- 1.2 for MII and 13.3 +/- 1.9 for MIII. The most frequent alteration detected in MI was loss on 22q (58%), In MII, aberrations most commonly identified were losses on 1p (76%), 22q (71%), 14q (43%), 18q (43%), 10 (38%), and 6q (33%), as well as gains on 20q (48%), 12q (43%), 15q (43%), 1q (33%), 9q (33%), and 17q (33%). In MIII, most of these alterations were found at similar frequencies, However, an increase in losses on 6q (53%), 10 (68%), and 14q (63%) was observed, In addition, 32% of MIII demonstrated loss on 9p, Homozygous deletions in the CDKV2A gene at 9p21 were found in 4 of 16 MIII (25%), Highly amplified DNA sequences were mapped to 12q13-q15 by CGH in 1 MII. Southern blot analysis of this tumor revealed amplification of CDK4 and MDM2. By CGH, DNA sequences from 17q were found to be amplified in 1 MII and 8 Mm; involving 17q23 in all cases, Despite the high frequency of chromosomal aberrations in the MII and Mm investigated, none of these tumors showed mutations in exons 5-8 of the TP53 gene, On the basis of the most common aberrations identified in the various malignancy grades, a model for the genomic alterations associated with meningioma progression is proposed.