Visualization of clustered IgE epitopes on α-lactalbumin

被引：49

作者：

Hochwallner, Heidrun ^{[2
]}

Schulmeister, Ulrike ^{[2
]}

Swoboda, Ines ^{[1
]}

Focke-Tejkl, Margit ^{[1
]}

Civaj, Vera ^{[1
]}

Balic, Nadja ^{[2
]}

Nystrand, Mats ^{[3
]}

Harlin, Annika ^{[3
]}

Thalhamer, Josef ^{[4
]}

Scheiblhofer, Sandra ^{[4
]}

Keller, Walter ^{[5
]}

Paykov, Tea ^{[5
]}

Zafred, Domen ^{[5
]}

Niggemann, Bodo ^{[6
]}

Quirce, Santiago ^{[7
]}

Mari, Adriano ^{[8
]}

Pauli, Gabrielle ^{[9
]}

Ebner, Christof ^{[10
]}

Papadopoulos, Nikolaos G. ^{[11
]}

Herz, Udo ^{[12
]}

van Tol, Eric A. F. ^{[12
]}

Valenta, Rudolf ^{[1
]}

Spitzauer, Susanne ^{[2
]}

机构：

[1] Med Univ Vienna, Christian Doppler Lab Allergy Res, Div Immunopathol, Dept Pathophysiol,Ctr Pathophysiol Infect & Immun, A-1090 Vienna, Austria

[2] Med Univ Vienna, Dept Med & Chem Lab Diagnost, A-1090 Vienna, Austria

[3] Phadia AB, Uppsala, Sweden

[4] Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, Dept Mol Biol, A-5020 Salzburg, Austria

[5] Graz Univ, Inst Mol Biosci, A-8010 Graz, Austria

[6] German Red Cross Hosp, Berlin, Germany

[7] Hosp La Paz, Dept Allergy, Madrid, Spain

[8] IRCCS, IDI, Ctr Clin & Expt Allergol, Rome, Italy

[9] Hop Univ, Serv Pneumol, Strasbourg, France

[10] Allergy Clin Reumannpl, Vienna, Austria

[11] Univ Athens, Dept Allergy, Pediat Clin 2, GR-10679 Athens, Greece

[12] Mead Johnson Nutr, Evansville, IN USA

来源：

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY | 2010年 / 125卷 / 06期

基金：

奥地利科学基金会;

关键词：

Allergen; food allergy; milk allergy; recombinant allergen; IgE epitope; GRASS-POLLEN ALLERGEN; IMMUNOGLOBULIN-E LEVELS; COWS MILK ALLERGY; BOVINE BETA-LACTOGLOBULIN; CROSS-REACTIVITY; SYNTHETIC PEPTIDES; BINDING; PROTEIN; DETERMINANTS; EXPRESSION;

D O I：

10.1016/j.jaci.2010.03.007

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Background: alpha-Lactalbumin (alpha-La) is a major cow's milk (CM) allergen responsible for allergic reactions in infants. Objective: We performed molecular, structural, and immunologic characterization of alpha-La. Methods: Recombinant alpha-lactalbumin (r alpha-La) was expressed in Escherichia coli, purified to homogeneity, and characterized by means of mass spectrometry and circular dichroism, and its allergenic activity was studied by using microarray technology, as well as in a basophil histamine release assay. IgE epitope mapping was performed with synthetic peptides. Results: According to circular dichroism analysis, rot-La represented a folded protein with a high thermal stability and refolding capacity. r alpha-La reacted with IgE antibodies from 57.6% of patients with CM allergy (n = 66) and induced the strongest basophil degranulation with sera from patients with CM allergy who had exhibited gastrointestinal symptoms or severe systemic reactions on CM exposure. r alpha-La contained sequential and conformational IgE epitopes. Superposition of IgE-reactive peptides onto the 3-dimensional structure of alpha-La revealed a close vicinity of the N- and C-terminal peptides within a surface-exposed patch. Conclusions: r alpha-La can be used for the diagnosis of patients with severe allergic reactions to CM and serves as a paradigmatic tool for the development of therapeutic strategies for CM allergy. (J Allergy Clin Immunol 2010;125:1279-85.)

引用

页码：1279 / 1285

页数：7

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