Development of an immunochromatographic test strip for the detection of papaverine in pure ginger powder

被引：11
作者：
Ge, Wenliang [1 ]
Suryoprabowo, Steven [2 ]
Zheng, Qiankun [3 ]
Kuang, Hua [2 ]
机构：
[1] Wuxi 2 Peoples Hosp, Wuxi, Peoples R China
[2] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[3] Delishi Grp, Weifang, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2017年 / 28卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Immunochromatographic assay; monoclonal antibody; papaverine; strip test; LIQUID-CHROMATOGRAPHIC DETERMINATION; MONOCLONAL-ANTIBODY; SENSITIVE DETECTION; BLOOD-SAMPLES; HPLC METHOD; VALIDATION; ALKALOIDS; PRODUCTS; PLASMA;
D O I：
10.1080/09540105.2017.1337086
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A leteral-flow immunochromatographic assay was developed for the detection of papaverine (PAPA) in pure ginger powder samples. We produced a sensitive monoclonal antibody againsts PAPA (anti-PAPA mAb) by immunizing BALB/c mice with a well-characterized PAPA-keyhole limpet hemocyanin conjugate, produced in our laboratory. The coating antigen (PAPA-ovalbumin) and goat anti-mouse IgG antibody were used as the capture reagent in the control line of the test strip. Under optimized conditions, the cut-off limits of the test strip was 1 ng/mL in 0.01 M PBS (pH 7.4) and 5 ng/mL in pure ginger powder. The results were obtained within 5 min. The results revealed that the developed method is a sensitive, rapid, and simple tool for the detection of PAPA in pure ginger powder.
引用
收藏
页码：1304 / 1314
页数：11
相关论文
共 25 条
[1]   Development and Validation of a Reversed-Phase HPLC Method for Determination of Alkaloids from Papaver somniferum L. (Papaveraceae) [J].
Acevska, Jelena ;
Dimitrovska, Aneta ;
Stefkov, Gjoshe ;
Brezovska, Katerina ;
Karapandzova, Marija ;
Kulevanova, Svetlana .
JOURNAL OF AOAC INTERNATIONAL, 2012, 95 (02) :399-405
[2]   Dispersive liquid-liquid microextraction followed by high-performance liquid chromatography-ultraviolet detection to determination of opium alkaloids in human plasma [J].
Ahmadi-Jouibari, Toraj ;
Fattahi, Nazir ;
Shamsipur, Mojtaba ;
Pirsaheb, Meghdad .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2013, 85 :14-20
[3]   DEVELOPMENT OF A NEW HPLC METHOD FOR DETERMINATION OF PAPAVERINE IN PRESENCE OF ITS PHOTOOXIDATION PRODUCTS [J].
Badea, Irinel A. ;
Vladescu, Luminita ;
David, Iulia G. ;
David, Vasile ;
Litescu, Simona C. .
ANALYTICAL LETTERS, 2010, 43 (7-8) :1217-1229
[4]   DETERMINATION OF PAPAVERINE IN BLOOD-SAMPLES BY GAS-CHROMATOGRAPHY USING A FLAME-IONIZATION AND A NITROGENPHOSPHORUS DETECTOR [J].
BELLIA, V ;
JACOB, J ;
SMITH, HT .
JOURNAL OF CHROMATOGRAPHY, 1978, 161 (NOV) :231-235
[5]   DETERMINATION OF PAPAVERINE IN BLOOD-SAMPLES BY GAS-LIQUID-CHROMATOGRAPHY AND MASS FRAGMENTOGRAPHY [J].
DEGRAEVE, J ;
VANCANTFORT, J ;
GIELEN, J .
JOURNAL OF CHROMATOGRAPHY, 1977, 133 (01) :153-160
[6]   Development and validation of a sandwich ELISA for quantification of peanut agglutinin (PNA) in foods [J].
Deng, Xiaofang ;
Liu, Liqiang ;
Ma, Wenwei ;
Xu, Chuanlai ;
Wang, Libing ;
Kuang, Hua .
FOOD AND AGRICULTURAL IMMUNOLOGY, 2012, 23 (03) :265-272
[7]   DETERMINATION OF PAPAVERINE AND COCAINE BY USE OF A PRECIPITATION SYSTEM COUPLED ONLINE TO AN ATOMIC-ABSORPTION SPECTROMETER [J].
EISMAN, M ;
GALLEGO, M ;
VARCARCEL, M .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1994, 12 (02) :179-184
[8]   DETERMINATION OF PAPAVERINE IN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
GAUTAM, SR ;
NAHUM, A ;
BAECHLER, J ;
BOURNE, DWA .
JOURNAL OF CHROMATOGRAPHY, 1980, 182 (3-4) :482-486
[9]   GLC DETERMINATION OF PAPAVERINE IN BIOLOGICAL-FLUIDS [J].
GUTTMAN, DE ;
KOSTENBAUDER, HB ;
WILKINSON, GR ;
DUBE, PH .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1974, 63 (10) :1625-1626
[10]   THIN-LAYER CHROMATOGRAPHIC METHOD FOR SEPARATION AND DETERMINATION OF PAPAVERINE AND ITS OXIDATION-PRODUCTS [J].
HABASHY, GM ;
FARID, NA .
TALANTA, 1973, 20 (07) :699-702
123