Construction and characterization of a live, attenuated esrB mutant of Edwardsiella tarda and its potential as a vaccine against the haemorrhagic septicaemia in turbot, Scophthamus maximus (L.)

被引：76

作者：

Lan, Mo Zhao

Peng, Xiao

Xiang, Mao Yun

Xia, Zou Yu

Bo, Wang

He, Li

Li, Xu Yong

Jun, Zhang Pei

机构：

[1] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

[2] Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Ocean Univ China, Qingdao 266003, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2007年 / 23卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Edwardsiella tarda; esrb mutant; live attenuated vaccine; turbot; Scophthamus maximus; AUXOTROPHIC AROA MUTANT; OUTER-MEMBRANE PROTEIN; AEROMONAS-HYDROPHILA; ESCHERICHIA-COLI; STRAINS; GENE; DETERMINANTS;

D O I：

10.1016/j.fsi.2006.11.002

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The esrB gene of Edwardsiella tarda, which encodes a regulator protein of the type III secretion system, was mutated by the unmarked deletion method and reintroduced by allelic exchange into the chromosome of E. tarda LSE40 by means of the suicide vector pRE 112. The LSE40 esrB mutant was highly attenuated when inoculated intraperitoneally into turbot Scophthamus maximus L., showing a 50% lethal dose of 10(8.1) cfu/fish. The esrB mutants were not recoverable from the internal organs at 14 days post-inoculation. Vaccination with a single dose of 10(5)-10(7) cfu/fish of the esrB mutant elicited significant protection against the wildtype strain of E. tarda LSE40 (relative percentage survival > 50%). The protection correlated well with the antibody titres in the serum of vaccinated fish. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：521 / 530

页数：10

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