A33scFv-cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy

被引：36

作者：

Deckert, PM ^{[1
]}

Renner, C

Cohen, LS

Jungbluth, A

Ritter, G

Bertino, JR

Old, LJ

Welt, S

机构：

[1] Free Univ Berlin, Klinikum Benjamin Franklin, Med Klin 3, D-12200 Berlin, Germany

[2] Univ Saarlandes Kliniken, Med Klin 1, D-66421 Homburg, Germany

[3] Ludwig Inst Canc Res, New York Branch, New York, NY 10021 USA

[4] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA

来源：

BRITISH JOURNAL OF CANCER | 2003年 / 88卷 / 06期

关键词：

tumour targeting; A33; antibody; antibody directed enzyme-producing therapy (ADEPT); colon carcinoma; recombinant fusion proteins;

D O I：

10.1038/sj.bjc.6600751

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A recombinant fusion protein of colon carcinoma binding A33 single chain antibody with cytosine deaminase displayed specific antigen binding and enzyme activity in surface plasmon resonance and is catalytic activity assay. In vitro, it selectively increased the toxicity of 5-FC to A33 antigen-positive cells by 300-fold, demonstrating the potency of this ADEPT strategy, (C) 2003 Cancer Research UK.

引用

页码：937 / 939

页数：3

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